ArticleJournal of visualized experiments : JoVE2013
Intravital microscopy for imaging subcellular structures in live mice expressing fluorescent proteins.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 18 citations in OpenAlex.
- Determination of Blood-Brain Barrier Hyperpermeability Using Intravital Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Intravital imaging to study cancer progression and metastasis.Nature reviews. Cancer · 2023Review
- Intravital microscopy of dynamic single-cell behavior in mouse mammary tissue.Nature protocols · 2021Article
- Actually Seeing What Is Going on - Intravital Microscopy in Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- Intravital Imaging Techniques for Biomedical and Clinical Research.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020Review
- Monitoring and Determining Mitochondrial Network Parameters in Live Lung Cancer Cells.Journal of clinical medicine · 2019Article
- Methods for imaging mammalian mitochondrial morphology: A prospective on MitoGraph.Analytical biochemistry · 2018Article
- Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy.Journal of visualized experiments : JoVE · 2018Article
- Deep tissue analysis of distal aqueous drainage structures and contractile features.Scientific reports · 2017Article
- Time-lapsed, large-volume, high-resolution intravital imaging for tissue-wide analysis of single cell dynamics.Methods (San Diego, Calif.) · 2017Article
- Live-cell imaging of ER-PM contact architecture by a novel TIRFM approach reveals extension of junctions in response to store-operated CaScientific reports · 2016Article
- A method for detailed analysis of the structure of mast cell secretory granules by negative contrast imaging.Scientific reports · 2016Article
- Multiscale modelling of saliva secretion.Mathematical biosciences · 2014Article
- In vivo tissue-wide synchronization of mitochondrial metabolic oscillations.Cell reports · 2014Article
- Probing the role of the actin cytoskeleton during regulated exocytosis by intravital microscopy.Methods in molecular biology (Clifton, N.J.) · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Here we describe a procedure to image subcellular structures in live rodents that is based on the use of confocal intravital microscopy. As a model organ, we use the salivary glands of live mice since they provide several advantages. First, they can be easily exposed to enable access to the optics, and stabilized to facilitate the reduction of the motion artifacts due to heartbeat and respiration. This significantly facilitates imaging and tracking small subcellular structures. Second, most of the cell populations of the salivary glands are accessible from the surface of the organ. This permits the use of confocal microscopy that has a higher spatial resolution than other techniques that have been used for in vivo imaging, such as two-photon microscopy. Finally, salivary glands can be easily manipulated pharmacologically and genetically, thus providing a robust system to investigate biological processes at a molecular level. In this study we focus on a protocol designed to follow the kinetics of the exocytosis of secretory granules in acinar cells and the dynamics of the apical plasma membrane where the secretory granules fuse upon stimulation of the beta-adrenergic receptors. Specifically, we used a transgenic mouse that co-expresses cytosolic GFP and a membrane-targeted peptide fused with the fluorescent protein tandem-Tomato. However, the procedures that we used to stabilize and image the salivary glands can be extended to other mouse models and coupled to other approaches to label in vivo cellular components, enabling the visualization of various subcellular structures, such as endosomes, lysosomes, mitochondria, and the actin cytoskeleton.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.