ArticlePloS one2013
RNA-Seq reveals infection-related gene expression changes in Phytophthora capsici.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- Validation of reference gene stability for normalization of RT-qPCR in Phytophthora capsici Leonian during its interaction with Piper nigrum L.Scientific reports · 2024Article
- Transcriptomic analysis reveals pathogenicity mechanisms ofFrontiers in microbiology · 2024Article
- Distinct small RNAs are expressed at different stages ofFrontiers in genetics · 2024Article
- Time-Course Transcriptome Profiling Reveals Differential Resistance Responses of Tomato to a Phytotoxic Effector of the Pathogenic OomycetePlants (Basel, Switzerland) · 2023Article
- Deciphering the underlying immune network of the potato defense response inhibition byFrontiers in plant science · 2023Article
- Functional analysis of the Nep1-like proteins fromPlant signaling & behavior · 2022Article
- Unraveling Plant Cell Death duringMicroorganisms · 2022Review
- Strive or thrive: Trends inFrontiers in plant science · 2022Article
- Differing Responses toFrontiers in plant science · 2022Article
- Genome Analysis ofPlants (Basel, Switzerland) · 2021Article
- High-Quality Reference Genome Sequence for the Oomycete Vegetable Pathogen Phytophthora capsici Strain LT1534.Microbiology resource announcements · 2021Article
- Cinnamaldehyde inhibits the growth ofPeerJ · 2021Article
- Functional Markers for Precision Plant Breeding.International journal of molecular sciences · 2020Review
- The transcriptional reprograming and functional identification of WRKY family members in pepper's response to Phytophthora capsici infection.BMC plant biology · 2020Article
- Differential Potential ofMicroorganisms · 2020Article
- Insight Into Function and Subcellular Localization ofFrontiers in microbiology · 2020Article
- Biocontrol and Action Mechanism ofInternational journal of molecular sciences · 2019Article
- A Phytophthora Effector Suppresses Trans-Kingdom RNAi to Promote Disease Susceptibility.Cell host & microbe · 2019Article
- Draft Genome Sequence for the Tree Pathogen Phytophthora plurivora.Genome biology and evolution · 2018Article
- Identification and functional analysis of the NLP-encoding genes from the phytopathogenic oomycete Phytophthora capsici.Molecular genetics and genomics : MGG · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytophthora capsici is a soilborne plant pathogen capable of infecting a wide range of plants, including many solanaceous crops. However, genetic resistance and fungicides often fail to manage P. capsici due to limited knowledge on the molecular biology and basis of P. capsici pathogenicity. To begin to rectify this situation, Illumina RNA-Seq was used to perform massively parallel sequencing of three cDNA samples derived from P. capsici mycelia (MY), zoospores (ZO) and germinating cysts with germ tubes (GC). Over 11 million reads were generated for each cDNA library analyzed. After read mapping to the gene models of P. capsici reference genome, 13,901, 14,633 and 14,695 putative genes were identified from the reads of the MY, ZO and GC libraries, respectively. Comparative analysis between two of samples showed major differences between the expressed gene content of MY, ZO and GC stages. A large number of genes associated with specific stages and pathogenicity were identified, including 98 predicted effector genes. The transcriptional levels of 19 effector genes during the developmental and host infection stages of P. capsici were validated by RT-PCR. Ectopic expression in Nicotiana benthamiana showed that P. capsici RXLR and Crinkler effectors can suppress host cell death triggered by diverse elicitors including P. capsici elicitin and NLP effectors. This study provides a first look at the transcriptome and effector arsenal of P. capsici during the important pre-infection stages.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.