ArticleThe Journal of biological chemistry2013
Structure and biosynthesis of two exopolysaccharides produced by Lactobacillus johnsonii FI9785.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 124 citations in OpenAlex.
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- Progress and challenges of functionalized bacterial encapsulation: A novel biotechnology for next-generation biotherapeutics.Acta pharmaceutica Sinica. B · 2025Review
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- Next-Generation Probiotics and Chronic Diseases: A Review of Current Research and Future Directions.Journal of agricultural and food chemistry · 2024Review
- Characterization of Probiotic Properties and Whole-Genome Analysis ofMicroorganisms · 2024Article
- Biological activities and applications of exopolysaccharides produced by lactic acid bacteria: a mini-review.World journal of microbiology & biotechnology · 2023Review
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- Antimicrobial activity against Staphylococcus aureus and genome features of Lactiplantibacillus plantarum LR-14 from Sichuan pickles.Archives of microbiology · 2022Article
- Biological Functions of Exopolysaccharides from Lactic Acid Bacteria and Their Potential Benefits for Humans and Farmed Animals.Foods (Basel, Switzerland) · 2022Review
- Biosynthesis of exopolysaccharide and structural characterization byFood chemistry. Molecular sciences · 2021Article
- Expression of genes involved in exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses.Archives of microbiology · 2021Article
- Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods.Foods (Basel, Switzerland) · 2021Review
- Decoding the Capability ofACS omega · 2020Article
- Potential Health Benefits of Ropy Exopolysaccharides Produced byMolecules (Basel, Switzerland) · 2020Article
- Application of microbial extracellular carbohydrate polymeric substances in food and allied industries.3 Biotech · 2020Review
- Identification of Genes Required for Glucan Exopolysaccharide Production in Lactobacillus johnsonii Suggests a Novel Biosynthesis Mechanism.Applied and environmental microbiology · 2020Article
- Galacto-Oligosaccharides Modulate the Juvenile Gut Microbiome and Innate Immunity To Improve Broiler Chicken Performance.mSystems · 2020Article
- Structural and technological characterization of ropy exopolysaccharides produced byFood science and biotechnology · 2020Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
Exopolysaccharides were isolated and purified from Lactobacillus johnsonii FI9785, which has previously been shown to act as a competitive exclusion agent to control Clostridium perfringens in poultry. Structural analysis by NMR spectroscopy revealed that L. johnsonii FI9785 can produce two types of exopolysaccharide: EPS-1 is a branched dextran with the unusual feature that every backbone residue is substituted with a 2-linked glucose unit, and EPS-2 was shown to have a repeating unit with the following structure: -6)-α-Glcp-(1-3)-β-Glcp-(1-5)-β-Galf-(1-6)-α-Glcp-(1-4)-β-Galp-(1-4)-β-Glcp-(1-. Sites on both polysaccharides were partially occupied by substituent groups: 1-phosphoglycerol and O-acetyl groups in EPS-1 and a single O-acetyl group in EPS-2. Analysis of a deletion mutant (ΔepsE) lacking the putative priming glycosyltransferase gene located within a predicted eps gene cluster revealed that the mutant could produce EPS-1 but not EPS-2, indicating that epsE is essential for the biosynthesis of EPS-2. Atomic force microscopy confirmed the localization of galactose residues on the exterior of wild type cells and their absence in the ΔepsE mutant. EPS2 was found to adopt a random coil structural conformation. Deletion of the entire 14-kb eps cluster resulted in an acapsular mutant phenotype that was not able to produce either EPS-2 or EPS-1. Alterations in the cell surface properties of the EPS-specific mutants were demonstrated by differences in binding of an anti-wild type L. johnsonii antibody. These findings provide insights into the biosynthesis and structures of novel exopolysaccharides produced by L. johnsonii FI9785, which are likely to play an important role in biofilm formation, protection against harsh environment of the gut, and colonization of the host.
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