ArticleProceedings of the National Academy of Sciences of the United States of America2013
Complete mitochondrial genome sequence of a Middle Pleistocene cave bear reconstructed from ultrashort DNA fragments.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 671 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
671 citing papers in PubMed.
- Article
- Ancient DNA and Human Physiology.Physiology (Bethesda, Md.) · 2026Review
- Comparing the Performance of Double-Stranded and Single-Stranded DNA Libraries for Ancient Oral Microbiome Reconstruction.Molecular ecology resources · 2026Article
- Population genomics, demography, and circum-Baltic connectivity of Early Medieval southwestern Finland.Genome biology · 2026Article
- Whole-Genome Sequencing Revealed Local Genetic Differences and Multi-Layered Genetic Structure in Asian Brown Bear (Ursus arctos) Populations.Genome biology and evolution · 2026Article
- Evaluation of bone preparation approaches using length-based analysis and targeted sequencing for forensic human identification of historic skeletal remains.Journal of forensic sciences · 2026Article
- Museum specimens reveal the genomic consequences of long-term population decline in an insect pollinator.Molecular biology and evolution · 2026Article
- Ancient wheat DNA: How and what for?Journal, genetic engineering & biotechnology · 2026Article
- Sedimentary Ancient DNA Tracks Multi-Kingdom Ecosystem Reorganizations Following Sequential Human Land Use at Crawford Lake.Molecular ecology · 2026Article
- The Oral Microbiome of King Richard III of England.American journal of biological anthropology · 2026Article
- Minimally invasive skeletal sampling for STR-based human identification in degraded marine remains.International journal of legal medicine · 2026Article
- Lifestyle Impacts the Oral Microbiome of Classical and Post-Classical Societies in Italy.American journal of biological anthropology · 2026Article
- Genetic differentiation and adaptive evolution of buff-tailed bumblebees in Asia.Insect science · 2026Article
- De novo assembly and authentication of ancient DNA metagenomes with nf-core/mag.PLoS computational biology · 2026Article
- A damage-aware NGS workflow for conservative species identification from ultra-degraded DNA.Analytical and bioanalytical chemistry · 2026Article
- Herbariomic approach solved identity crisis of the putatively extinct Armeria arcuata Welw. ex Boiss. & Reut. (Plumbaginaceae).Annals of botany · 2026Article
- Genomic insights into the Iron Age Saka of Boz-Barmak, Kyrgyzstan.Scientific reports · 2026Article
- Ancient mitogenomes reveal the genetic relationship among extinct and extant water buffaloes.iScience · 2026Article
- Multidisciplinary Exploration of Computed-Tomographic and Ancient-DNA Data of an Iron Age Skull From Latvia With Multiple Lytic Bone Lesions: Differential Diagnosis Between Metastatic Carcinoma, Multiple Myeloma and Skeletal Tuberculosis.Ecology and evolution · 2026Article
- Article
611 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although an inverse relationship is expected in ancient DNA samples between the number of surviving DNA fragments and their length, ancient DNA sequencing libraries are strikingly deficient in molecules shorter than 40 bp. We find that a loss of short molecules can occur during DNA extraction and present an improved silica-based extraction protocol that enables their efficient retrieval. In combination with single-stranded DNA library preparation, this method enabled us to reconstruct the mitochondrial genome sequence from a Middle Pleistocene cave bear (Ursus deningeri) bone excavated at Sima de los Huesos in the Sierra de Atapuerca, Spain. Phylogenetic reconstructions indicate that the U. deningeri sequence forms an early diverging sister lineage to all Western European Late Pleistocene cave bears. Our results prove that authentic ancient DNA can be preserved for hundreds of thousand years outside of permafrost. Moreover, the techniques presented enable the retrieval of phylogenetically informative sequences from samples in which virtually all DNA is diminished to fragments shorter than 50 bp.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.