ArticleProceedings of the National Academy of Sciences of the United States of America2013
A chemostat array enables the spatio-temporal analysis of the yeast proteome.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
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- Subcellular Transcriptomics and Proteomics: A Comparative Methods Review.Molecular & cellular proteomics : MCP · 2022Review
- Mesostats-A multiplexed, low-cost, do-it-yourself continuous culturing system for experimental evolution of mesocosms.PloS one · 2022Article
- Copy Number Analysis of the Yeast Histone Deacetylase Complex Component Cti6 Directly in Living Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Proximity-Dependent Biotinylation Approaches to Explore the Dynamic Compartmentalized Proteome.Frontiers in molecular biosciences · 2022Review
- Single-cell image analysis to explore cell-to-cell heterogeneity in isogenic populations.Cell systems · 2021Review
- Subcellular proteomics.Nature reviews. Methods primers · 2021Article
- TheCellVision.org: A Database for Visualizing and Mining High-Content Cell Imaging Projects.G3 (Bethesda, Md.) · 2020Article
- Genome-Wide Dynamic Evaluation of the UV-Induced DNA Damage Response.G3 (Bethesda, Md.) · 2020Article
- Pby1 is a direct partner of the Dcp2 decapping enzyme.Nucleic acids research · 2020Article
- DeLTA: Automated cell segmentation, tracking, and lineage reconstruction using deep learning.PLoS computational biology · 2020Article
- Genome-scale transcriptional dynamics and environmental biosensing.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Isolating live cells after high-throughput, long-term, time-lapse microscopy.Nature methods · 2020Article
- Pinpointing Cell Identity in Time and Space.Frontiers in molecular biosciences · 2020Article
- Incorporation of a unified protein abundance dataset into the Saccharomyces genome database.Database : the journal of biological databases and curation · 2020Article
- Frontiers in Microfluidics, a Teaching Resource Review.Bioengineering (Basel, Switzerland) · 2019Review
- Enhancement of LacI binding in vivo.Nucleic acids research · 2019Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Observing cellular responses to perturbations is central to generating and testing hypotheses in biology. We developed a massively parallel microchemostat array capable of growing and observing 1,152 yeast-GFP strains on the single-cell level with 20 min time resolution. We measured protein abundance and localization changes in 4,085 GFP-tagged strains in response to methyl methanesulfonate and analyzed 576 GFP strains in five additional conditions for a total of more than 10,000 unique experiments, providing a systematic view of the yeast proteome in flux. We observed that processing bodies formed rapidly and synchronously in response to UV irradiation, and in conjunction with 506 deletion-GFP strains, identified four gene disruptions leading to abnormal ribonucleotide-diphosphate reductase (Rnr4) localization. Our microchemostat platform enables the large-scale interrogation of proteomes in flux and permits the concurrent observation of protein abundance, localization, cell size, and growth parameters on the single-cell level for thousands of microbial cultures in one experiment.
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