ArticleMolecular & cellular proteomics : MCP2013
Identification of BZR1-interacting proteins as potential components of the brassinosteroid signaling pathway in Arabidopsis through tandem affinity purification.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 83 citations in OpenAlex.
- Brachypodium HISTONE DEACETYLASE 19.1 promotes yield, flowering, and freezing tolerance at the expense of drought tolerance.BMC plant biology · 2026Article
- UPL3 promotes BZR1 degradation, growth arrest, and seedling survival under starvation stress in Arabidopsis.Plant communications · 2025Article
- Calmodulin-Binding Transcription Factors: Roles in Plant Response to Abiotic Stresses.Plants (Basel, Switzerland) · 2025Review
- Structural and functional characterization of cellulose synthase proteins (CesA) in rice and their regulation via brassinosteroid signaling under arsenate stress.Plant cell reports · 2024Article
- Mechanistic Insights into the Function of 14-3-3 Proteins as Negative Regulators of Brassinosteroid Signaling in Arabidopsis.Plant & cell physiology · 2024Article
- Brassinosteroid biosynthesis and signaling: Conserved and diversified functions of core genes across multiple plant species.Plant communications · 2024Review
- Transcription factors BZR1 and PAP1 cooperate to promote anthocyanin biosynthesis in Arabidopsis shoots.The Plant cell · 2024Article
- The BAS chromatin remodeler determines brassinosteroid-induced transcriptional activation and plant growth in Arabidopsis.Developmental cell · 2024Article
- A Revised View of theInternational journal of molecular sciences · 2023Review
- The Role of Calmodulin Binding Transcription Activator in Plants under Different Stressors: Physiological, Biochemical, Molecular Mechanisms ofBioengineering (Basel, Switzerland) · 2022Review
- Deubiquitination of BES1 by UBP12/UBP13 promotes brassinosteroid signaling and plant growth.Plant communications · 2022Article
- GhBZR3 suppresses cotton fiber elongation by inhibiting very-long-chain fatty acid biosynthesis.The Plant journal : for cell and molecular biology · 2022Article
- Deubiquitinating enzymes UBP12 and UBP13 stabilize the brassinosteroid receptor BRI1.EMBO reports · 2022Article
- Comprehensive genome-wide analysis of calmodulin-binding transcription activator (CAMTA) in Durio zibethinus and identification of fruit ripening-associated DzCAMTAs.BMC genomics · 2021Article
- OsWAK112, A Wall-Associated Kinase, Negatively Regulates Salt Stress Responses by Inhibiting Ethylene Production.Frontiers in plant science · 2021Article
- Role of Protein Phosphatase1 Regulatory Subunit3 in Mediating the Abscisic Acid Response.Plant physiology · 2020Article
- Chromatin Regulation in the Response of Ethylene: Nuclear Events in Ethylene Signaling.Small methods · 2020Article
- CaFrontiers in plant science · 2020Review
- Mutual Regulation of Receptor-Like Kinase SIT1 and B'κ-PP2A Shapes the Early Response of Rice to Salt Stress.The Plant cell · 2019Article
- TTL Proteins Scaffold Brassinosteroid Signaling Components at the Plasma Membrane to Optimize Signal Transduction in Arabidopsis.The Plant cell · 2019Article
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
Abstract
Brassinosteroids (BRs) are essential phytohormones for plant growth and development. BRs are perceived by the cell surface receptor kinase BRI1, and downstream signal transduction through multiple components leads to activation of the transcription factors BZR1 and BZR2/BES1. BZR1 activity is highly controlled by BR through reversible phosphorylation, protein degradation, and nucleocytoplasmic shuttling. To further understand the molecular function of BZR1, we performed tandem affinity purification of the BZR1 complex and identified BZR1-associated proteins using mass spectrometry. These BZR1-associated proteins included several known BR signaling components, such as BIN2, BSK1, 14-3-3λ, and PP2A, as well as a large number of proteins with previously unknown functions in BR signal transduction, including the kinases MKK5 and MAPK4, histone deacetylase 19, cysteine proteinase inhibitor 6, a DEAD-box RNA helicase, cysteine endopeptidases RD21A and RD21B, calmodulin-binding transcription activator 5, ubiquitin protease 12, cyclophilin 59, and phospholipid-binding protein synaptotagmin A. Their interactions with BZR1 were confirmed by in vivo and in vitro assays. Furthermore, MKK5 was found to phosphorylate BZR1 in vitro. This study demonstrates an effective method for purifying proteins associated with low-abundance transcription factors, and identifies new BZR1-interacting proteins with potentially important roles in BR response.
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