ArticleStem cells translational medicine2013
Isolation of human adipose-derived stromal cells using laser-assisted liposuction and their therapeutic potential in regenerative medicine.
Article in Stem cells translational medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Intra-articular injection of autologous adipose-derived stromal vascular fractions for knee osteoarthritis: a double-blind randomized self-controlled trial.International orthopaedics · 2019Trial
- An Update on Adipose-Derived Stem Cells for Regenerative Medicine: Where Challenge Meets Opportunity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- A Bibliometric and Visualization Analysis on the Research of Fat Grafting from 1945 to 2021.Aesthetic plastic surgery · 2023Review
- Musculoskeletal tissue engineering: Adipose derived stromal cell implementation for the treatment of osteoarthritis.Biomaterials · 2022Review
- Extracellular Vesicles of Mesenchymal Stromal Cells Can be Taken Up by Microglial Cells and Partially Prevent the Stimulation Induced by β-amyloid.Stem cell reviews and reports · 2022Article
- Adipose-Derived Stem Cells for Facial Rejuvenation.Journal of personalized medicine · 2022Review
- [Current status and prospects of clinical application of liposuction].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2022Review
- Lipofilling in Breast Oncological Surgery: A Safe Opportunity or Risk for Cancer Recurrence?International journal of molecular sciences · 2021Review
- Cannula Size Effect on Stromal Vascular Fraction Content of Fat Grafts.Plastic and reconstructive surgery. Global open · 2021Article
- Comparative Analysis of Two Automated Fat-processing Systems.Plastic and reconstructive surgery. Global open · 2020Article
- Hypoxia Rapidly Induces the Expression of Cardiomyogenic Factors in Human Adipose-Derived Adherent Stromal Cells.Journal of clinical medicine · 2019Article
- Periostin expression and characters of human adipose tissue-derived mesenchymal stromal cells were aberrantly affected byStem cell investigation · 2019Article
- In Vitro and In Vivo Osteogenic Differentiation of Human Adipose-Derived Stromal Cells.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Autologous fat grafting: Harvesting techniques.Annals of medicine and surgery (2012) · 2018Review
- Review: Proposed Methods to Improve the Survival of Adipose Tissue in Autologous Fat Grafting.Plastic and reconstructive surgery. Global open · 2018Article
- Characteristics and Immunomodulating Functions of Adipose-Derived and Bone Marrow-Derived Mesenchymal Stem Cells Across Defined Human Leukocyte Antigen Barriers.Frontiers in immunology · 2018Article
- The Use of Vibrational Energy to Isolate Adipose-Derived Stem Cells.Plastic and reconstructive surgery. Global open · 2018Article
- Fibrin Glue Enhances Adipose-Derived Stromal Cell Cytokine Secretion and Survival Conferring Accelerated Diabetic Wound Healing.Stem cells international · 2018Article
- Ultrasound-assisted liposuction provides a source for functional adipose-derived stromal cells.Cytotherapy · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
18 authors.
Funding
Abstract
Harvesting adipose-derived stromal cells (ASCs) for tissue engineering is frequently done through liposuction. However, several different techniques exist. Although third-generation ultrasound-assisted liposuction has been shown to not have a negative effect on ASCs, the impact of laser-assisted liposuction on the quality and differentiation potential of ASCs has not been studied. Therefore, ASCs were harvested from laser-assisted lipoaspirate and suction-assisted lipoaspirate. Next, in vitro parameters of cell yield, cell viability and proliferation, surface marker phenotype, osteogenic differentiation, and adipogenic differentiation were performed. Finally, in vivo bone formation was assessed using a critical-sized cranial defect in athymic nude mice. Although ASCs isolated from suction-assisted lipoaspirate and laser-assisted lipoaspirate both successfully underwent osteogenic and adipogenic differentiation, the cell yield, viability, proliferation, and frequency of ASCs (CD34(+)CD31(-)CD45(-)) in the stromal vascular fraction were all significantly less with laser-assisted liposuction in vitro (p < .05). In vivo, quantification of osseous healing by micro-computed tomography revealed significantly more healing with ASCs isolated from suction-assisted lipoaspirate relative to laser-assisted lipoaspirate at the 4-, 6-, and 8-week time points (p < .05). Therefore, as laser-assisted liposuction appears to negatively impact the biology of ASCs, cell harvest using suction-assisted liposuction is preferable for tissue-engineering purposes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.