ArticleCell calcium2013
Synthesis and properties of Asante Calcium Red--a novel family of long excitation wavelength calcium indicators.
Article in Cell calcium, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Improved Workflow for Analysis of Vascular Myocyte Time-Series and Line-Scan CaInternational journal of molecular sciences · 2023Article
- Fluorescent Indicators For Biological Imaging of Monatomic Ions.Frontiers in cell and developmental biology · 2022Review
- Control of hearing sensitivity by tectorial membrane calcium.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F.Frontiers in cell and developmental biology · 2019Article
- Optical Probes for Neurobiological Sensing and Imaging.Accounts of chemical research · 2018Review
- A comparison of fluorescent Ca²⁺ indicators for imaging local Ca²⁺ signals in cultured cells.Cell calcium · 2015Article
- Deep two-photon brain imaging with a red-shifted fluorometric Ca2+ indicator.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Asante Calcium Green and Asante Calcium Red--novel calcium indicators for two-photon fluorescence lifetime imaging.PloS one · 2014Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Although many synthetic calcium indicators are available, a search for compounds with improved characteristics continues. Here, we describe the synthesis and properties of Asante Calcium Red-1 (ACR-1) and its low affinity derivative (ACR-1-LA) created by linking BAPTA to seminaphthofluorescein. The indicators combine a visible light (450-540 nm) excitation with deep-red fluorescence (640 nm). Upon Ca2+ binding, the indicators raise their fluorescence with longer excitation wavelengths producing higher responses. Although the changes occur without any spectral shifts, it is possible to ratio Ca(2+)-dependent (640 nm) and quasi-independent (530 nm) emission when using visible (< 490 nm) or multiphoton (∼780 nm) excitation. Therefore, both probes can be used as single wavelength or, less dynamic, ratiometric indicators. Long indicator emission might allow easy [Ca2+]i measurement in GFP expressing cells. The indicators bind Ca2+ with either high (Kd = 0.49 ± 0.07 μM; ACR-1) or low affinity (Kd = 6.65 ± 0.13 μM; ACR-1-LA). Chelating Zn2+ (Kd = 0.38 ± 0.02 nM) or Mg2+ (Kd∼5mM) slightly raises and binding Co2+ quenches dye fluorescence. New indicators are somewhat pH-sensitive (pKa = 6.31 ± 0.07), but fairly resistant to bleaching. The probes are rather dim, which combined with low AM ester loading efficiency, might complicate in situ imaging. Despite potential drawbacks, ACR-1 and ACR-1-LA are promising new calcium indicators.
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