ArticleExperimental eye research2013
Presence of native limbal stromal cells increases the expansion efficiency of limbal stem/progenitor cells in culture.
Article in Experimental eye research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 59 citations in OpenAlex.
- Article
- Review
- The Spatial Transcriptomic Atlas of Human Limbus and Vital Niche Microenvironment Regulating the Fate of Limbal Epithelial Stem Cells.Investigative ophthalmology & visual science · 2025Article
- Review
- Proinflammatory gene and protein expression alterations in human limbal aniridia fibroblasts.PloS one · 2025Article
- Progress in Transdifferentiation of Autologous Alternative Cell Sources into Corneal Epithelial Cells.Stem cell reviews and reports · 2025Review
- Targeting limbal epithelial stem cells: master conductors of corneal epithelial regeneration from the bench to multilevel theranostics.Journal of translational medicine · 2024Review
- Immunophenotypical Characterization of Limbal Mesenchymal Stromal Cell Subsets during In Vitro Expansion.International journal of molecular sciences · 2024Article
- Wnt activation as a potential therapeutic approach to treat partial limbal stem cell deficiency.Scientific reports · 2023Article
- Concise Review: Bioengineering of Limbal Stem Cell Niche.Bioengineering (Basel, Switzerland) · 2023Review
- Review
- Isolation andBio-protocol · 2022Article
- Efficient Isolation and Functional Characterization of Niche Cells from Human Corneal Limbus.International journal of molecular sciences · 2022Article
- Human limbal epithelial stem cell regulation, bioengineering and function.Progress in retinal and eye research · 2021Review
- Wnt6 plays a complex role in maintaining human limbal stem/progenitor cells.Scientific reports · 2021Article
- The Limbal Niche and Regenerative Strategies.Vision (Basel, Switzerland) · 2021Review
- Current perspectives of limbal-derived stem cells and its application in ocular surface regeneration and limbal stem cell transplantation.Stem cells translational medicine · 2021Review
- Stem Cell Niche Microenvironment: Review.Bioengineering (Basel, Switzerland) · 2021Review
- A Comparison of Methods for Isolation of Limbal Niche Cells: Maintenance of Limbal Epithelial Stem/Progenitor Cells.Investigative ophthalmology & visual science · 2020Article
- Expansion of Human Limbal Epithelial Stem/Progenitor Cells Using Different Human Sera: A Multivariate Statistical Analysis.International journal of molecular sciences · 2020Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Niche factors are important in the maintenance and regulation of stem cells. Limbal stromal cells are potentially a component of limbal stem cell (LSC) niche. We investigated the role of the limbal stromal cells in the ex vivo expansion of limbal stem/progenitor cells. Limbal epithelial cells were cultured as single-cell suspension and cell clusters from dispase II or collagenase A (ColA), or tissue explant. ColA isolated limbal stromal cells along with limbal epithelial cells. In the presence of limbal stromal cells, a higher absolute number of p63α(bright) cells (p < 0.05) and a higher proportion of K14 positive epithelial cells were obtained from both ColA and explant tissue cultures. Expansion of the stem/progenitor population from dispase isolation was more efficient in the form of cell clusters than single cell suspension based on the absolute number of p63α(bright) cells. Expansion of the stem cell population is similar in the single cell and cell cluster cultures that are derived from ColA isolation. Our finding suggests that limbal stromal cells and an intact cell-cell contact help to maintain LSCs in an undifferentiated state in vitro during expansion.
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