ArticleCellular microbiology2014
The Toxoplasma gondii kinetochore is required for centrosome association with the centrocone (spindle pole).
Article in Cellular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 44 citations in OpenAlex.
- A modular chromosomal passenger complex rewires chromosome segregation in Plasmodium berghei.Nature communications · 2026Article
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- An apical ring protein essential for conoid complex assembly and daughter cell formation in Toxoplasma gondii.Nature communications · 2025Article
- The PP2A-2 holoenzyme orchestrates daughter cell emergence during cytokinesis in Toxoplasma gondii.PLoS pathogens · 2025Article
- Deciphering cell cycle organization ofmBio · 2025Article
- DivergentLife science alliance · 2025Article
- Disruption of Plasmodium falciparum kinetochore proteins destabilises the nexus between the centrosome equivalent and the mitotic apparatus.Nature communications · 2024Article
- Balancing Act: Tubulin Glutamylation and Microtubule Dynamics inMicroorganisms · 2024Review
- The SUN-like protein TgSLP1 is essential for nuclear division in the apicomplexan parasite Toxoplasma gondii.Journal of cell science · 2023Article
- Article
- Composition and organization of kinetochores show plasticity in apicomplexan chromosome segregation.The Journal of cell biology · 2022Article
- Restriction Checkpoint Controls Bradyzoite Development in Toxoplasma gondii.Microbiology spectrum · 2022Article
- Structural and Functional Insights into the Microtubule Organizing Centers ofMicroorganisms · 2021Review
- Article
- The Modular Circuitry of Apicomplexan Cell Division Plasticity.Frontiers in cellular and infection microbiology · 2021Review
- Real-time dynamics ofJournal of cell science · 2020Article
- A Homolog of Structural Maintenance of Chromosome 1 Is a Persistent Centromeric Protein Which Associates With Nuclear Pore Components inFrontiers in cellular and infection microbiology · 2020Article
- Fussing About Fission: Defining Variety Among Mainstream and Exotic Apicomplexan Cell Division Modes.Frontiers in cellular and infection microbiology · 2020Article
- Microtubule number and length determine cellular shape and function in Plasmodium.The EMBO journal · 2019Article
- Toxoplasma F-box protein 1 is required for daughter cell scaffold function during parasite replication.PLoS pathogens · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The kinetochore is a multi-protein structure assembled on eukaryotic centromeres mediating chromosome attachment to spindle microtubules. Here we identified the kinetochore proteins Nuf2 and Ndc80 in the apicomplexan parasite Toxoplasma gondii. Localization revealed that kinetochores remain clustered throughout the cell cycle and colocalize with clustered centromeres at the centrocone, a structure containing the spindle pole embedded in the nuclear envelope. Pharmacological disruption of microtubules resulted in partial loss of some kinetochore and centromere clustering, indicating microtubules are necessary but not strictly required for kinetochore clustering. Generation of a TgNuf2 conditional knock-down strain revealed it is essential for chromosome segregation, but dispensable for centromere clustering. The centromeres actually remained associated with the centrocone suggesting microtubule binding is not required for their interaction with the spindle pole. The most striking observation upon TgNuf2 depletion was that the centrosome behaved normally, but that it lost its association with the centrocone. This suggests that microtubules are essential to maintain contact between the centrosome and chromosomes, and this interaction is critical for the partitioning of the nuclei into the two daughter parasites. Finally, genetic complementation experiments with mutated TgNuf2 constructs highlighted an apicomplexan-specific motif with a putative role in nuclear localization.
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