ArticleeLife2013
Cytoplasmic dynein crosslinks and slides anti-parallel microtubules using its two motor domains.
Article in eLife, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 82 citations in OpenAlex.
- Nuclear Entanglement: New Insights Into the Role of Cytoskeleton and Nucleoskeleton in Plant Nuclear Function.Cytoskeleton (Hoboken, N.J.) · 2026Review
- Motor-Assisted Co-Migration of Intracellular Organelles and Microtubules as a Mechanism for Directed Cargo Transport.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- NuMA mechanically reinforces the spindle independently of its partner dynein.Current biology : CB · 2025Article
- NuMA mechanically reinforces the spindle independently of its partner dynein.bioRxiv : the preprint server for biology · 2024Article
- Microtubule choreography: spindle self-organization during cell division.Biophysical reviews · 2024Review
- A circle of life: platelet and megakaryocyte cytoskeleton dynamics in health and disease.Open biology · 2024Review
- Reconstitution of Neuronal Motor Traffic on Septin-Associated Microtubules.Methods in molecular biology (Clifton, N.J.) · 2024Article
- NudC regulated Lis1 stability is essential for the maintenance of dynamic microtubule ends in axon terminals.iScience · 2022Article
- Review
- Article
- Don't you forget about me(gakaryocytes).Blood · 2022Article
- Septins guide noncentrosomal microtubules to promote focal adhesion disassembly in migrating cells.Molecular biology of the cell · 2022Article
- Force by minus-end motors Dhc1 and Klp2 collapses the S. pombe spindle after laser ablation.Biophysical journal · 2022Article
- Article
- Novel mechanism for oscillations in catchbonded motor-filament complexes.Biophysical journal · 2021Article
- A septin GTPase scaffold of dynein-dynactin motors triggers retrograde lysosome transport.The Journal of cell biology · 2021Article
- Mechanical Regulation of Neurite Polarization and Growth: A Computational Study.Biophysical journal · 2020Article
- Kinesin-5 Eg5 is essential for spindle assembly and chromosome alignment of mouse spermatocytes.Cell division · 2020Article
- Septin 2/6/7 complexes tune microtubule plus-end growth and EB1 binding in a concentration- and filament-dependent manner.Molecular biology of the cell · 2019Article
- Unconventional Roles of Cytoskeletal Mitotic Machinery in Neurodevelopment.Trends in cell biology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Cytoplasmic dynein is the predominant minus-end-directed microtubule (MT) motor in most eukaryotic cells. In addition to transporting vesicular cargos, dynein helps to organize MTs within MT networks such as mitotic spindles. How dynein performs such non-canonical functions is unknown. Here we demonstrate that dynein crosslinks and slides anti-parallel MTs in vitro. Surprisingly, a minimal dimeric motor lacking a tail domain and associated subunits can cause MT sliding. Single molecule imaging reveals that motors pause and frequently reverse direction when encountering an anti-parallel MT overlap, suggesting that the two motor domains can bind both MTs simultaneously. In the mitotic spindle, inward microtubule sliding by dynein counteracts outward sliding generated by kinesin-5, and we show that a tailless, dimeric motor is sufficient to drive this activity in mammalian cells. Our results identify an unexpected mechanism for dynein-driven microtubule sliding, which differs from filament sliding mechanisms described for other motor proteins. DOI:http://dx.doi.org/10.7554/eLife.00943.001.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.