ArticlePloS one2013
Topical gene electrotransfer to the epidermis of hairless guinea pig by non-invasive multielectrode array.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Intradermal DNA vaccine delivery using vacuum-controlled, needle-free electroporation.Molecular therapy. Nucleic acids · 2023Article
- Real-time impedance feedback to enhance cutaneous gene electrotransfer in a murine skin model.Bioelectrochemistry (Amsterdam, Netherlands) · 2021Article
- Moderate Heat-Assisted Gene Electrotransfer as a Potential Delivery Approach for Protein Replacement Therapy through the Skin.Pharmaceutics · 2021Article
- Moderate Heat-Assisted Gene Electrotransfer for Cutaneous Delivery of a DNA Vaccine Against Hepatitis B Virus.Human gene therapy · 2021Article
- Synergistic effects of an atmospheric pressure plasma jet and pulsed electric field on cells and skin.IEEE transactions on plasma science. IEEE Nuclear and Plasma Sciences Society · 2021Article
- Coalesced thermal and electrotransfer mediated delivery of plasmid DNA to the skin.Bioelectrochemistry (Amsterdam, Netherlands) · 2019Article
- Safe and efficient novel approach for non-invasive gene electrotransfer to skin.Scientific reports · 2018Article
- Electroporation as a vaccine delivery system and a natural adjuvant to intradermal administration of plasmid DNA in macaques.Scientific reports · 2017Article
- Electrotransfer parameters as a tool for controlled and targeted gene expression in skin.Molecular therapy. Nucleic acids · 2016Article
- Recellularized human dermis for testing gene electrotransfer ex vivo.Biomedical materials (Bristol, England) · 2016Article
- Perspectives on Transdermal Electroporation.Pharmaceutics · 2016Review
- Thermal Assisted In Vivo Gene Electrotransfer.Current gene therapy · 2016Article
- Improved Specificity of Gene Electrotransfer to Skin Using pDNA Under the Control of Collagen Tissue-Specific Promoter.The Journal of membrane biology · 2015Article
- Application of increased temperature from an exogenous source to enhance gene electrotransfer.Bioelectrochemistry (Amsterdam, Netherlands) · 2015Article
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Authors and funding
5 authors.
Funding
Abstract
Topical gene delivery to the epidermis has the potential to be an effective therapy for skin disorders, cutaneous cancers, vaccinations and systemic metabolic diseases. Previously, we reported on a non-invasive multielectrode array (MEA) that efficiently delivered plasmid DNA and enhanced expression to the skin of several animal models by in vivo gene electrotransfer. Here, we characterized plasmid DNA delivery with the MEA in a hairless guinea pig model, which has a similar histology and structure to human skin. Significant elevation of gene expression up to 4 logs was achieved with intradermal DNA administration followed by topical non-invasive skin gene electrotransfer. This delivery produced gene expression in the skin of hairless guinea pig up to 12 to 15 days. Gene expression was observed exclusively in the epidermis. Skin gene electrotransfer with the MEA resulted in only minimal and mild skin changes. A low level of human Factor IX was detected in the plasma of hairless guinea pig after gene electrotransfer with the MEA, although a significant increase of Factor IX was obtained in the skin of animals. These results suggest gene electrotransfer with the MEA can be a safe, efficient, non-invasive skin delivery method for skin disorders, vaccinations and potential systemic diseases where low levels of gene products are sufficient.
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Registered trials
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