Evidence map›Paper›PMID 24015301›Full record

ArticlePloS one2013

Cardiomyocyte protection by GATA-4 gene engineered mesenchymal stem cells is partially mediated by translocation of miR-221 in microvesicles.

Bin Yu, Min Gong, Yigang Wang, Ronald W Millard, Zeeshan Pasha, Yueting Yang, Muhammad Ashraf, Meifeng Xu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
78citing papers in PubMed, 3 pooled it
4.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

78 citing papers in PubMed, 3 syntheses or guidelines pooled it, 130 citations in OpenAlex.

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  19. Mesenchymal Stem Cells and Their Exocytotic Vesicles.International journal of molecular sciences · 2023
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18 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Bin YuDepartment of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, Ohio, United States of America.
Min Gong
Yigang Wang
Ronald W Millard
Zeeshan Pasha
Yueting Yang
Muhammad Ashraf
Meifeng Xu
University of Cincinnati Medical Center · USUniversity of Cincinnati · US

Funding

MicroRNA as mediators of angiogenesis & ischemic myocardial repairR01HL114654 · NHLBI · UNIVERSITY OF CINCINNATI · PI ASHRAF, MUHAMMAD, XU, MEIFENG · 2012 to 2016
$2.5M
Wnt11 signaling in stem cell survival and cardiac regenerationR01HL105176 · NHLBI · UNIVERSITY OF CINCINNATI · PI ASHRAF, MUHAMMAD, JONES, WALTER KEITH · 2010 to 2014
$1.9M
NHLBI NIH HHS HL105176NHLBI NIH HHS HL114654NHLBI NIH HHS R01 HL105176NHLBI NIH HHS R01 HL114654
6 · The paper itself

Abstract

introductionmicroRNAs (miRs), a novel class of small non-coding RNAs, are involved in cell proliferation, differentiation, development, and death. In this study, we found that miR-221 translocation by microvesicles (MVs) plays an important role in cardioprotection mediated by GATA-4 overexpressed mesenchymal stem cells (MSC). METHODS AND

resultsAdult rat bone marrow MSC and neonatal rat ventricle cardiomyocytes (CM) were harvested as primary cultures. MSC were transduced with GATA-4 (MSC(GATA-4)) using the murine stem cell virus (pMSCV) retroviral expression system. Empty vector transfection was used as a control (MSC(Null)). The expression of miRs was assessed by real-time PCR and localized using in situ hybridization (ISH). MVs collected from MSC cultures were characterized by expression of CD9, CD63, and HSP70, and photographed with electron microscopy. Cardioprotection during hypoxia afforded by conditioned medium (CdM) from MSC cultures was evaluated by lactate dehydrogenase (LDH) release, MTS uptake by CM, and caspase 3/7 activity. Expression of miR-221/222 was significantly higher in MSC than in CM and miR-221 was upregulated in MSC(GATA-4). MSC overexpression of miR-221 significantly enhanced cardioprotection by reducing the expression of p53 upregulated modulator of apoptosis (PUMA). Moreover, expression of PUMA was significantly decreased in CM co-cultured with MSC. MVs derived from MSC expressed high levels of miR-221, and were internalized quickly by CM as documented in images obtained from a Time-Lapse Imaging System.

conclusionsOur results demonstrate that cardioprotection by MSC(GATA-4) may be regulated in part by a transfer of anti-apoptotic miRs contained within MVs.

Indexed as

AnimalsApoptosis Regulatory ProteinsCell HypoxiaCoculture TechniquesGATA4 Transcription FactorHeart VentriclesMaleMesenchymal Stem CellsMicroRNAsMyocytes, CardiacRatsRats, Sprague-DawleyTumor Suppressor Protein p53Apoptosis Regulatory ProteinsBbc3 protein, ratGata4 protein, ratGATA4 Transcription FactorMicroRNAsMIRN221 microRNA, ratTumor Suppressor Protein p53

Identifiers

PMID24015301
PMCPMC3756018
OpenAlexW2054272773

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.