ArticleMicrobiology (Reading, England)2013
A synthetic system for expression of components of a bacterial microcompartment.
Article in Microbiology (Reading, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Changing surface grafting density has an effect on the activity of immobilized xylanase towards natural polysaccharides.Scientific reports · 2019Article
- In Vitro Assembly of Diverse Bacterial Microcompartment Shell Architectures.Nano letters · 2018Article
- Programmed loading and rapid purification of engineered bacterial microcompartment shells.Nature communications · 2018Article
- Engineering nanoreactors using bacterial microcompartment architectures.Current opinion in biotechnology · 2018Review
- Bacterial microcompartments.Nature reviews. Microbiology · 2018Review
- Transfer and analysis of Salmonella pdu genes in a range of Gram-negative bacteria demonstrate exogenous microcompartment expression across a variety of species.Microbial biotechnology · 2018Article
- Facile methods for heterologous production of bacterial microcompartments in diverse host species.Microbial biotechnology · 2018Review
- Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli.Microbial cell factories · 2017Article
- Cellular factories for coenzyme QMicrobial cell factories · 2017Review
- Effect of bio-engineering on size, shape, composition and rigidity of bacterial microcompartments.Scientific reports · 2016Article
- Building Spatial Synthetic Biology with Compartments, Scaffolds, and Communities.Cold Spring Harbor perspectives in biology · 2016Review
- Employing bacterial microcompartment technology to engineer a shell-free enzyme-aggregate for enhanced 1,2-propanediol production in Escherichia coli.Metabolic engineering · 2016Article
- Engineering formation of multiple recombinant Eut protein nanocompartments in E. coli.Scientific reports · 2016Article
- Exploring bacterial organelle interactomes: a model of the protein-protein interaction network in the Pdu microcompartment.PLoS computational biology · 2015Article
- Diverse bacterial microcompartment organelles.Microbiology and molecular biology reviews : MMBR · 2014Review
Corrections and comments
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Authors and funding
17 authors at 2 institutions in 1 country.
Funding
Abstract
In general, prokaryotes are considered to be single-celled organisms that lack internal membrane-bound organelles. However, many bacteria produce proteinaceous microcompartments that serve a similar purpose, i.e. to concentrate specific enzymic reactions together or to shield the wider cytoplasm from toxic metabolic intermediates. In this paper, a synthetic operon encoding the key structural components of a microcompartment was designed based on the genes for the Salmonella propanediol utilization (Pdu) microcompartment. The genes chosen included pduA, -B, -J, -K, -N, -T and -U, and each was shown to produce protein in an Escherichia coli chassis. In parallel, a set of compatible vectors designed to express non-native cargo proteins was also designed and tested. Engineered hexa-His tags allowed isolation of the components of the microcompartments together with co-expressed, untagged, cargo proteins. Finally, an in vivo protease accessibility assay suggested that a PduD-GFP fusion could be protected from proteolysis when co-expressed with the synthetic microcompartment operon. This work gives encouragement that it may be possible to harness the genes encoding a non-native microcompartment for future biotechnological applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.