ArticleApplied and environmental microbiology2013
C1 metabolism in Corynebacterium glutamicum: an endogenous pathway for oxidation of methanol to carbon dioxide.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 62 citations in OpenAlex.
- Multi-omics analyses of evolvedSynthetic and systems biotechnology · 2026Article
- Deciphering the molecular mechanism and regulation of formaldehyde detoxification inApplied and environmental microbiology · 2024Article
- Engineering of the DNA replication and repair machinery to develop binary mutators for rapid genome evolution of Corynebacterium glutamicum.Nucleic acids research · 2023Article
- Metabolic engineering strategies for microbial utilization of methanol.Engineering microbiology · 2023Review
- Molecular dissection of a dedicated formaldehyde dehydrogenase from Mycobacterium smegmatis.Protein science : a publication of the Protein Society · 2022Article
- Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy.Microorganisms · 2022Review
- A Novel NADP-Dependent Formate Dehydrogenase From the Hyperthermophilic ArchaeonFrontiers in microbiology · 2022Article
- FNR-Type Regulator GoxR of the Obligatorily Aerobic Acetic Acid BacteriumApplied and environmental microbiology · 2021Article
- Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae.Nature communications · 2020Article
- Deciphering the crucial roles of AraC-type transcriptional regulator Cgl2680 on NADPH metabolism and L-lysine production in Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2020Article
- Adaptive laboratory evolution enhances methanol tolerance and conversion in engineered Corynebacterium glutamicum.Communications biology · 2020Article
- Recent Progress on Chemical Production From Non-food Renewable Feedstocks UsingFrontiers in bioengineering and biotechnology · 2020Review
- Article
- Methylotrophy in Mycobacteria: Dissection of the Methanol Metabolism Pathway in Mycobacterium smegmatis.Journal of bacteriology · 2018Article
- S-nitrosomycothiol reductase and mycothiol are required for survival under aldehyde stress and biofilm formation in Mycobacterium smegmatis.IUBMB life · 2016Article
- Identification of two mutations increasing the methanol tolerance of Corynebacterium glutamicum.BMC microbiology · 2015Article
- Metabolic engineering of Corynebacterium glutamicum for methanol metabolism.Applied and environmental microbiology · 2015Article
- Metabolic pathway engineering for production of 1,2-propanediol and 1-propanol by Corynebacterium glutamicum.Biotechnology for biofuels · 2015Article
- Redox regulation by reversible protein S-thiolation in bacteria.Frontiers in microbiology · 2015Review
- Characterization of p-hydroxycinnamate catabolism in a soil Actinobacterium.Journal of bacteriology · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methanol is considered an interesting carbon source in "bio-based" microbial production processes. Since Corynebacterium glutamicum is an important host in industrial biotechnology, in particular for amino acid production, we performed studies of the response of this organism to methanol. The C. glutamicum wild type was able to convert (13)C-labeled methanol to (13)CO2. Analysis of global gene expression in the presence of methanol revealed several genes of ethanol catabolism to be upregulated, indicating that some of the corresponding enzymes are involved in methanol oxidation. Indeed, a mutant lacking the alcohol dehydrogenase gene adhA showed a 62% reduced methanol consumption rate, indicating that AdhA is mainly responsible for methanol oxidation to formaldehyde. Further studies revealed that oxidation of formaldehyde to formate is catalyzed predominantly by two enzymes, the acetaldehyde dehydrogenase Ald and the mycothiol-dependent formaldehyde dehydrogenase AdhE. The Δald ΔadhE and Δald ΔmshC deletion mutants were severely impaired in their ability to oxidize formaldehyde, but residual methanol oxidation to CO2 was still possible. The oxidation of formate to CO2 is catalyzed by the formate dehydrogenase FdhF, recently identified by us. Similar to the case with ethanol, methanol catabolism is subject to carbon catabolite repression in the presence of glucose and is dependent on the transcriptional regulator RamA, which was previously shown to be essential for expression of adhA and ald. In conclusion, we were able to show that C. glutamicum possesses an endogenous pathway for methanol oxidation to CO2 and to identify the enzymes and a transcriptional regulator involved in this pathway.
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