ArticleApplied and environmental microbiology2013
BopA does not have a major role in the adhesion of Bifidobacterium bifidum to intestinal epithelial cells, extracellular matrix proteins, and mucus.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 38 citations in OpenAlex.
- Viable and Heat-InactivatedMicroorganisms · 2026Article
- Artificial polysaccharide-binding protein mediates co-assembly of nanodrug and probiotics against drug-resistant infectious enteritis.Asian journal of pharmaceutical sciences · 2025Article
- Review
- Genome-wide siRNA screening reveals several host receptors for the binding of human gut commensal Bifidobacterium bifidum.NPJ biofilms and microbiomes · 2022Article
- Critical roles of a housekeeping sortase of probioticiScience · 2021Article
- The metabolic profile of Bifidobacterium dentium reflects its status as a human gut commensal.BMC microbiology · 2021Article
- The Infant-DerivedMicroorganisms · 2020Article
- Isolation of Anti-Inflammatory and Epithelium ReinforcingNutrients · 2020Article
- NovelFrontiers in microbiology · 2020Article
- Microbiota and Cancer: The Emerging Beneficial Role of Bifidobacteria in Cancer Immunotherapy.Frontiers in microbiology · 2020Review
- Review
- Effects ofMicroorganisms · 2019Article
- Bifidobacterial Dialogue With Its Human Host and Consequent Modulation of the Immune System.Frontiers in immunology · 2019Review
- Growth Mode and Carbon Source Impact the Surfaceome Dynamics ofFrontiers in microbiology · 2019Article
- Article
- Pili-like proteins of Akkermansia muciniphila modulate host immune responses and gut barrier function.PloS one · 2017Article
- Long-term effects on luminal and mucosal microbiota and commonly acquired taxa in faecal microbiota transplantation for recurrent Clostridium difficile infection.BMC medicine · 2016Article
- Proteinaceous Molecules Mediating Bifidobacterium-Host Interactions.Frontiers in microbiology · 2016Review
- Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism ofFrontiers in microbiology · 2016Article
- A Critical Evaluation of Bifidobacterial Adhesion to the Host Tissue.Frontiers in microbiology · 2016Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of bifidobacteria to adhere to the intestine of the human host is considered to be important for efficient colonization and achieving probiotic effects. Bifidobacterium bifidum strains DSM20456 and MIMBb75 adhere well to the human intestinal cell lines Caco-2 and HT-29. The surface lipoprotein BopA was previously described to be involved in mediating adherence of B. bifidum to epithelial cells, but thioacylated, purified BopA inhibited the adhesion of B. bifidum to epithelial cells in competitive adhesion assays only at very high concentrations, indicating an unspecific effect. In this study, the role of BopA in the adhesion of B. bifidum was readdressed. The gene encoding BopA was cloned and expressed without its lipobox and hydrophobic signal peptide in Escherichia coli, and an antiserum against the recombinant BopA was produced. The antiserum was used to demonstrate the abundant localization of BopA on the cell surface of B. bifidum. However, blocking of B. bifidum BopA with specific antiserum did not reduce adhesion of bacteria to epithelial cell lines, arguing that BopA is not an adhesin. Also, adhesion of B. bifidum to human colonic mucin and fibronectin was found to be BopA independent. The recombinant BopA bound only moderately to human epithelial cells and colonic mucus, and it failed to bind to fibronectin. Thus, our results contrast the earlier findings on the major role of BopA in adhesion, indicating that the strong adhesion of B. bifidum to epithelial cell lines is BopA independent.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.