ArticleApplied and environmental microbiology2013
Isolation of butanol- and isobutanol-tolerant bacteria and physiological characterization of their butanol tolerance.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 75 citations in OpenAlex.
- Integrating synthetic biology and process engineering for enhanced isobutanol biosynthesis yield and sustainability.Biotechnology for biofuels and bioproducts · 2026Review
- Bacterial Tolerance to 1-Butanol and 2-Butanol: Quantitative Assessment and Transcriptomic Response.International journal of molecular sciences · 2024Article
- Microbial host engineering for sustainable isobutanol production from renewable resources.Applied microbiology and biotechnology · 2024Review
- Transcriptional and post-transcriptional mechanisms modulate cyclopropane fatty acid synthase through small RNAs inJournal of bacteriology · 2024Article
- Control ofMetabolites · 2021Article
- Increasing Solvent Tolerance to Improve Microbial Production of Alcohols, Terpenoids and Aromatics.Microorganisms · 2021Review
- Genomics and transcriptomics analysis reveals the mechanism of isobutanol tolerance of a laboratory evolved Lactococcus lactis strain.Scientific reports · 2020Article
- To beat the heat - engineering of the most thermostable pyruvate decarboxylase to date.RSC advances · 2019Article
- Streamlined assessment of membrane permeability and its application to membrane engineering of Escherichia coli for octanoic acid tolerance.Journal of industrial microbiology & biotechnology · 2019Article
- Tolerance against butanol stress by disrupting succinylglutamate desuccinylase inRSC advances · 2019Article
- Low cell surface hydrophobicity is one of the key factors for high butanol tolerance of Lactic acid bacteria.Engineering in life sciences · 2019Article
- Growth of Pseudomonas taiwanensis VLB120∆C biofilms in the presence of n-butanol.Microbial biotechnology · 2017Article
- Growth inhibition of S. cerevisiae, B. subtilis, and E. coli by lignocellulosic and fermentation products.Applied microbiology and biotechnology · 2016Article
- Engineering membrane and cell-wall programs for tolerance to toxic chemicals: Beyond solo genes.Current opinion in microbiology · 2016Review
- pH-induced change in cell susceptibility to butanol in a high butanol-tolerant bacterium, Enterococcus faecalis strain CM4A.Biotechnology for biofuels · 2015Article
- A Comparison of the Microbial Production and Combustion Characteristics of Three Alcohol Biofuels: Ethanol, 1-Butanol, and 1-Octanol.Frontiers in bioengineering and biotechnology · 2015Review
- Dynamic transition of chemolithotrophic sulfur-oxidizing bacteria in response to amendment with nitrate in deposited marine sediments.Frontiers in microbiology · 2015Article
- Isolation of microorganisms involved in reduction of crystalline iron(III) oxides in natural environments.Frontiers in microbiology · 2015Article
- An oleaginous bacterium that intrinsically accumulates long-chain free Fatty acids in its cytoplasm.Applied and environmental microbiology · 2014Article
- Genetic resources for advanced biofuel production described with the Gene Ontology.Frontiers in microbiology · 2014Review
Corrections and comments
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite their importance as a biofuel production platform, only a very limited number of butanol-tolerant bacteria have been identified thus far. Here, we extensively explored butanol- and isobutanol-tolerant bacteria from various environmental samples. A total of 16 aerobic and anaerobic bacteria that could tolerate greater than 2.0% (vol/vol) butanol and isobutanol were isolated. A 16S rRNA gene sequencing analysis revealed that the isolates were phylogenetically distributed over at least nine genera: Bacillus, Lysinibacillus, Rummeliibacillus, Brevibacillus, Coprothermobacter, Caloribacterium, Enterococcus, Hydrogenoanaerobacterium, and Cellulosimicrobium, within the phyla Firmicutes and Actinobacteria. Ten of the isolates were phylogenetically distinct from previously identified butanol-tolerant bacteria. Two relatively highly butanol-tolerant strains CM4A (aerobe) and GK12 (obligate anaerobe) were characterized further. Both strains changed their membrane fatty acid composition in response to butanol exposure, i.e., CM4A and GK12 exhibited increased saturated and cyclopropane fatty acids (CFAs) and long-chain fatty acids, respectively, which may serve to maintain membrane fluidity. The gene (cfa) encoding CFA synthase was cloned from strain CM4A and expressed in Escherichia coli. The recombinant E. coli showed relatively higher butanol and isobutanol tolerance than E. coli without the cfa gene, suggesting that cfa can confer solvent tolerance. The exposure of strain GK12 to butanol by consecutive passages even enhanced the growth rate, indicating that yet-unknown mechanisms may also contribute to solvent tolerance. Taken together, the results demonstrate that a wide variety of butanol- and isobutanol-tolerant bacteria that can grow in 2.0% butanol exist in the environment and have various strategies to maintain structural integrity against detrimental solvents.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.