ArticlePloS one2013
Glycine insertion makes yellow fluorescent protein sensitive to hydrostatic pressure.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 26 citations in OpenAlex.
- Rapid changes in hydrostatic pressure as a probe for correlating function of purified proteins with their measured activity in living cells.Biophysical reviews · 2025Review
- Glycine insertion modulates the fluorescence properties ofBiophysics and physicobiology · 2021Article
- Session 1SHA-control of biological functions with hydrostatic pressure stimulation.Biophysical reviews · 2020Article
- Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification.International journal of molecular sciences · 2019Review
- In vivo analysis of protein crowding within the nuclear pore complex in interphase and mitosis.Scientific reports · 2017Article
- Design and development of genetically encoded fluorescent sensors to monitor intracellular chemical and physical parameters.Biophysical reviews · 2016Review
- Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it.Scientific reports · 2016Article
- Flowers under pressure: ins and outs of turgor regulation in development.Annals of botany · 2014Review
- Single-molecule analysis of the rotation of F₁-ATPase under high hydrostatic pressure.Biophysical journal · 2013Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent protein-based indicators for intracellular environment conditions such as pH and ion concentrations are commonly used to study the status and dynamics of living cells. Despite being an important factor in many biological processes, the development of an indicator for the physicochemical state of water, such as pressure, viscosity and temperature, however, has been neglected. We here found a novel mutation that dramatically enhances the pressure dependency of the yellow fluorescent protein (YFP) by inserting several glycines into it. The crystal structure of the mutant showed that the tyrosine near the chromophore flipped toward the outside of the β-can structure, resulting in the entry of a few water molecules near the chromophore. In response to changes in hydrostatic pressure, a spectrum shift and an intensity change of the fluorescence were observed. By measuring the fluorescence of the YFP mutant, we succeeded in measuring the intracellular pressure change in living cell. This study shows a new strategy of design to engineer fluorescent protein indicators to sense hydrostatic pressure.
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