ArticleThe Journal of biological chemistry2013
The thiamine biosynthetic enzyme ThiC catalyzes multiple turnovers and is inhibited by S-adenosylmethionine (AdoMet) metabolites.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
- Thiamin addition to soil increases potato tuber thiamin content under greenhouse conditions.PeerJ · 2026Article
- A [2.1.0]-Fused Bicyclic Intermediate Is Produced during the Biosynthesis of Oxetane Nucleosides.Journal of the American Chemical Society · 2025Article
- Comparative Genomic and Genetic Evidence on a Role for the OarX Protein in Thiamin Salvage.ACS omega · 2024Article
- OsTH1 is a key player in thiamin biosynthesis in rice.Scientific reports · 2024Article
- Why pyridoxal phosphate could be a functional predecessor of thiamine pyrophosphate and speculations on a primordial metabolism.RSC chemical biology · 2024Review
- Thiamine-Mediated Microbial Interaction between Auxotrophic Rhodococcus ruber ZM07 and Prototrophic Cooperators in the Tetrahydrofuran-Degrading Microbial Community H-1.Microbiology spectrum · 2023Article
- On the Evolutionary History of the Twenty Encoded Amino Acids.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Review
- Thiamine metabolism genes in diatoms are not regulated by thiamine despite the presence of predicted riboswitches.The New phytologist · 2022Article
- Natural Variation in Vitamin BFrontiers in plant science · 2022Article
- Thiamine Is Required for Virulence and Survival ofFrontiers in microbiology · 2022Article
- The Moderately (D)efficient Enzyme: Catalysis-Related DamageBiochemistry · 2021Article
- Functional characterization of the HMP-P synthase of Legionella pneumophila (Lpg1565).Molecular microbiology · 2021Article
- TheFrontiers in plant science · 2021Article
- Microbial Cell Factories for Green Production of Vitamins.Frontiers in bioengineering and biotechnology · 2021Review
- The l-Thr Kinase/l-Thr-Phosphate Decarboxylase (CobD) Enzyme fromBiochemistry · 2019Article
- Investigation of ( S)-(-)-Acidomycin: A Selective Antimycobacterial Natural Product That Inhibits Biotin Synthase.ACS infectious diseases · 2019Article
- Salvage of the 5-deoxyribose byproduct of radical SAM enzymes.Nature communications · 2018Article
- C-C bond forming radical SAM enzymes involved in the construction of carbon skeletons of cofactors and natural products.Natural product reports · 2018Review
- Toward Eradication of B-Vitamin Deficiencies: Considerations for Crop Biofortification.Frontiers in plant science · 2018Review
- Perturbation of the metabolic network in Salmonella enterica reveals cross-talk between coenzyme A and thiamine pathways.PloS one · 2018Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
ThiC (4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase; EC 4.1.99.17) is a radical S-adenosylmethionine (AdoMet) enzyme that uses a [4Fe-4S](+) cluster to reductively cleave AdoMet to methionine and a 5'-deoxyadenosyl radical that initiates catalysis. In plants and bacteria, ThiC converts the purine intermediate 5-aminoimidazole ribotide to 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate, an intermediate of thiamine pyrophosphate (coenzyme B1) biosynthesis. In this study, assay conditions were implemented that consistently generated 5-fold molar excess of HMP, demonstrating that ThiC undergoes multiple turnovers. ThiC activity was improved by in situ removal of product 5'-deoxyadenosine. The activity was inhibited by AdoMet metabolites S-adenosylhomocysteine, adenosine, 5'-deoxyadenosine, S-methyl-5'-thioadenosine, methionine, and homocysteine. Neither adenosine nor S-methyl-5'-thioadenosine had been shown to inhibit radical AdoMet enzymes, suggesting that ThiC is distinct from other family members. The parameters for improved ThiC activity and turnover described here will facilitate kinetic and mechanistic analyses of ThiC.
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