ArticleThe Journal of biological chemistry2013
Assessing the conformational changes of pb5, the receptor-binding protein of phage T5, upon binding to its Escherichia coli receptor FhuA.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- From Cloning Vectors to Phage Display: Engineering Principles and Translational Applications of Bacteriophage Display Platforms.International journal of molecular sciences · 2026Review
- Bacteriophage-Based Therapeutic, Diagnostic, and Biocontrol Platforms: Engineering, Evidence, and Translational Challenges.International journal of molecular sciences · 2026Review
- Deciphering Bacteriophage T5 Host Recognition Mechanism and Infection Trigger.Journal of virology · 2023Article
- Structural basis of bacteriophage T5 infection trigger andScience advances · 2023Article
- Structural basis for host recognition and superinfection exclusion by bacteriophage T5.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Revisiting the host adhesion determinants of Streptococcus thermophilus siphophages.Microbial biotechnology · 2020Article
- Interdomain Flexibility within NADPH Oxidase Suggested by SANS Using LMNG Stealth Carrier.Biophysical journal · 2020Article
- Conserved and Diverse Traits of Adhesion Devices fromViruses · 2020Review
- Examining Membrane Proteins by Neutron Scattering.Methods in molecular biology (Clifton, N.J.) · 2020Article
- A Quest of Great Importance-Developing a Broad SpectrumViruses · 2019Article
- Targeting mechanisms of tailed bacteriophages.Nature reviews. Microbiology · 2018Review
- Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.Structure (London, England : 1993) · 2018Article
- Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water.PloS one · 2018Article
- Aberrantly Large Single-Channel Conductance of Polyhistidine Arm-Containing Protein Nanopores.Biochemistry · 2017Article
- Structure of the host-recognition device of Staphylococcus aureus phage ϕ11.Scientific reports · 2016Article
- Article
- Uniqueness of models from small-angle scattering data: the impact of a hydration shell and complementary NMR restraints.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Probing the conformation of FhaC with small-angle neutron scattering and molecular modeling.Biophysical journal · 2014Article
- Structures and host-adhesion mechanisms of lactococcal siphophages.Frontiers in microbiology · 2014Review
- Insights into bacteriophage T5 structure from analysis of its morphogenesis genes and protein components.Journal of virology · 2014Article
Corrections and comments
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Authors and funding
8 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Within tailed bacteriophages, interaction of the receptor-binding protein (RBP) with the target cell triggers viral DNA ejection into the host cytoplasm. In the case of phage T5, the RBP pb5 and the receptor FhuA, an outer membrane protein of Escherichia coli, have been identified. Here, we use small angle neutron scattering and electron microscopy to investigate the FhuA-pb5 complex. Specific deuteration of one of the partners allows the complete masking in small angle neutron scattering of the surfactant and unlabeled proteins when the complex is solubilized in the fluorinated surfactant F6-DigluM. Thus, individual structures within a membrane protein complex can be described. The solution structure of FhuA agrees with its crystal structure; that of pb5 shows an elongated shape. Neither displays significant conformational changes upon interaction. The mechanism of signal transduction within phage T5 thus appears different from that of phages binding cell wall saccharides, for which structural information is available.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.