ArticleNature methods2013
Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light.
Article in Nature methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 173 papers.
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Who cites it
173 citing papers in PubMed, 349 citations in OpenAlex.
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- Optimization of spatial and temporal sampling resolution for optophysiology in large-scale two-photon calcium imaging.Biomedical optics express · 2026Article
- High-speed whole-brain imaging in Drosophila.Nature communications · 2026Article
- Deep neural networks to register and annotate cells in moving and deforming nervous systems.eLife · 2026Article
- Light-field deep learning enables high-throughput, scattering-mitigated calcium imaging.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Integrative functional optical imaging approaches: Optics, microfluidics, and machine learning for neuroscience in organoids and small-animal models.Biophysical journal · 2025Review
- Network modularity reveals context and state-dependent reorganization of time-varying functional connectivity in single-cell resolved neural activity recordings.bioRxiv : the preprint server for biology · 2025Article
- Bridging the gap between the connectome and whole-brain activity inbioRxiv : the preprint server for biology · 2025Article
- Unifying community whole-brain imaging datasets enables robust neuron identification and reveals determinants of neuron position in C. elegans.Cell reports methods · 2025Article
- Review
- Out-of-focus signal rejection forBiomedical optics express · 2025Article
- CRASH2p: Closed-loop Two Photon Imaging in a Freely Moving Animal.bioRxiv : the preprint server for biology · 2024Article
- Neuro-cognitive multilevel causal modeling: A framework that bridges the explanatory gap between neuronal activity and cognition.PLoS computational biology · 2024Article
- Intricate response dynamics enhances stimulus discrimination in the resource-limited C. elegans chemosensory system.BMC biology · 2024Article
- Advanced Neural Functional Imaging inMicromachines · 2024Review
- Unifying community-wide whole-brain imaging datasets enables robust automated neuron identification and reveals determinants of neuron positioning inbioRxiv : the preprint server for biology · 2024Article
- C. elegans foraging as a model for understanding the neuronal basis of decision-making.Cellular and molecular life sciences : CMLS · 2024Review
- Building and integrating brain-wide maps of nervous system function in invertebrates.Current opinion in neurobiology · 2024Review
113 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent efforts in neuroscience research have been aimed at obtaining detailed anatomical neuronal wiring maps as well as information on how neurons in these networks engage in dynamic activities. Although the entire connectivity map of the nervous system of Caenorhabditis elegans has been known for more than 25 years, this knowledge has not been sufficient to predict all functional connections underlying behavior. To approach this goal, we developed a two-photon technique for brain-wide calcium imaging in C. elegans, using wide-field temporal focusing (WF-TeFo). Pivotal to our results was the use of a nuclear-localized, genetically encoded calcium indicator, NLS-GCaMP5K, that permits unambiguous discrimination of individual neurons within the densely packed head ganglia of C. elegans. We demonstrate near-simultaneous recording of activity of up to 70% of all head neurons. In combination with a lab-on-a-chip device for stimulus delivery, this method provides an enabling platform for establishing functional maps of neuronal networks.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.