ArticlePlant cell reports2013
Selection of reliable reference genes for quantitative real-time polymerase chain reaction studies in maize grains.
Article in Plant cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed.
- Selection and validation of reliable reference genes for quantitative real-time PCR in Barnyard millet (Echinochloa spp.) under varied abiotic stress conditions.Scientific reports · 2023Article
- Selection of suitable reference genes for qPCR normalization in different developmental stages ofFrontiers in plant science · 2023Article
- Gene Expression Analysis by Quantitative Real-Time PCR for Floral Tissues.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Analysis of controlling genes for tiller growth of Psathyrostachys juncea based on transcriptome sequencing technology.BMC plant biology · 2022Article
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- Uncovering the Gene Regulatory Network of Maize Hybrid ZD309 under Heat Stress by Transcriptomic and Metabolomic Analysis.Plants (Basel, Switzerland) · 2022Article
- Selection and Validation of Reference Genes for Quantitative Real-Time PCR Analysis of Development and Tissue-Dependent Flower Color Formation inInternational journal of molecular sciences · 2022Article
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- Evaluation of duplicated reference genes for quantitative real-time PCR analysis in genome unknown hexaploid oat (Plant methods · 2020Article
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- DiazotrophicFrontiers in microbiology · 2019Article
- Selection of suitable reference genes for qRT-PCR normalisation under different experimental conditions in Eucommia ulmoides Oliv.Scientific reports · 2018Article
- Validation of reference genes for RT-qPCR studies inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Evaluation of Biosynthesis, Accumulation and Antioxidant Activityof Vitamin E in Sweet Corn (Zea mays L.) during Kernel Development.International journal of molecular sciences · 2017Article
- The manipulation of gene expression and the biosynthesis of Vitamin C, E and folate in light-and dark-germination of sweet corn seeds.Scientific reports · 2017Article
- Effect of Light- and Dark-Germination on the Phenolic Biosynthesis, Phytochemical Profiles, and Antioxidant Activities in Sweet Corn (Zea mays L.) Sprouts.International journal of molecular sciences · 2017Article
- Stable Internal Reference Genes for Normalizing Real-Time Quantitative PCR inFrontiers in plant science · 2017Article
- LbCML38 and LbRH52, two reference genes derived from RNA-Seq data suitable for assessing gene expression in Lycium barbarum L.Scientific reports · 2016Article
- Selection of suitable reference genes for qRT-PCR normalization during leaf development and hormonal stimuli in tea plant (Camellia sinensis).Scientific reports · 2016Article
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4 authors.
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Abstract
key messageThe stability of candidate reference genes was evaluated in maize landrace varieties and during multiple grain developmental stages to evaluate the expression of carotenoid-related genes by RT-qPCR for application to maize biofortification. Vitamin A deficiency affects millions of children worldwide; therefore, increasing the content of vitamin A precursors in maize grains is of interest. The study of the expression of genes involved in the carotenoid biosynthetic pathway in maize grains has provided useful information for metabolic engineering approaches. However, reliable results using real-time quantitative polymerase chain reaction (RT-qPCR) experiments are dependent on the use of the appropriate reference genes. In this study, we utilized geNorm and NormFinder softwares to identify the most stably expressed candidate reference genes in samples from seven stages of grain development and from eight landrace varieties. The results of the analysis performed using geNorm indicated that tubulin (TUB) and actin (ACT) were the most suitable reference genes among all experimental conditions, while glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH) showed the least stability. The same result was obtained with the NormFinder software. The minimum number of genes required in each experimental condition to normalize the gene expression data was also determined by geNorm. The expression of phytoene synthase gene (PSY1), the first enzyme in the carotenoid biosynthetic pathway, was overestimated when the least stable candidate gene (GAPDH) was used as the internal control instead of the most stable gene pair (ACT + TUB), thus highlighting the importance of validating reference genes before conducting a RT-qPCR experiment to obtain accurate results. This study is the first survey of the stability of genes for use as reference genes to normalize RT-qPCR data from maize landraces during multiple stages of grain development.
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