ArticleNucleic acids research2013
Mechanical properties of DNA-like polymers.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Approaches for Determining DNA Persistence Length Using Atomic Force Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Predictions of DNA mechanical properties at a genomic scale reveal potentially new functional roles of DNA flexibility.NAR genomics and bioinformatics · 2023Article
- Superanionic DNA: enzymatic synthesis of hypermodified DNA bearing four different anionic substituents at all four nucleobases.Nucleic acids research · 2023Article
- Nanomechanics of negatively supercoiled diaminopurine-substituted DNA.Nucleic acids research · 2021Article
- A Study on the Bending Stiffness of a New DNA Origami Nano-Joint.Molecular biotechnology · 2021Article
- Synthesis of Polyanionic C5-Modified 2'-Deoxyuridine and 2'-Deoxycytidine-5'-Triphosphates and Their Properties as Substrates for DNA Polymerases.Molecules (Basel, Switzerland) · 2021Article
- Structure-mechanics statistical learning unravels the linkage between local rigidity and global flexibility in nucleic acids.Chemical science · 2020Article
- Article
- Nanomechanics of Diaminopurine-Substituted DNA.Biophysical journal · 2019Article
- Comparative analysis of inosine-substituted duplex DNA by circular dichroism and X-ray crystallography.Journal of biomolecular structure & dynamics · 2018Article
- Nanostructures from Synthetic Genetic Polymers.Chembiochem : a European journal of chemical biology · 2016Article
- The structural diversity of artificial genetic polymers.Nucleic acids research · 2016Review
- Article
- Nonantibiotic Effects of Fluoroquinolones in Mammalian Cells.The Journal of biological chemistry · 2015Article
- Oxygen concentration controls epigenetic effects in models of familial paraganglioma.PloS one · 2015Article
- Effect of temperature on the intrinsic flexibility of DNA and its interaction with architectural proteins.Biochemistry · 2014Article
- DNA, flexibly flexible.Biophysical journal · 2014Article
- Mechanical properties of base-modified DNA are not strictly determined by base stacking or electrostatic interactions.Biophysical journal · 2014Article
- The free solution mobility of DNA and other analytes varies as the logarithm of the fractional negative charge.Electrophoresis · 2014Article
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5 authors.
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Abstract
The molecular structure of the DNA double helix has been known for 60 years, but we remain surprisingly ignorant of the balance of forces that determine its mechanical properties. The DNA double helix is among the stiffest of all biopolymers, but neither theory nor experiment has provided a coherent understanding of the relative roles of attractive base stacking forces and repulsive electrostatic forces creating this stiffness. To gain insight, we have created a family of double-helical DNA-like polymers where one of the four normal bases is replaced with various cationic, anionic or neutral analogs. We apply DNA ligase-catalyzed cyclization kinetics experiments to measure the bending and twisting flexibilities of these polymers under low salt conditions. Interestingly, we show that these modifications alter DNA bending stiffness by only 20%, but have much stronger (5-fold) effects on twist flexibility. We suggest that rather than modifying DNA stiffness through a mechanism easily interpretable as electrostatic, the more dominant effect of neutral and charged base modifications is their ability to drive transitions to helical conformations different from canonical B-form DNA.
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