ArticlePloS one2013
Expression profiling of Plasmodium berghei HSP70 genes for generation of bright red fluorescent parasites.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 29 citations in OpenAlex.
- Expanding the fluorescent toolkit: Blue fluorescent protein-expressing Plasmodium berghei for enhanced multiplex microscopy.PloS one · 2025Article
- Avian malaria in a feral-pet pigeon: a case report.Malaria journal · 2024Article
- A member of the tryptophan-rich protein family is required for efficient sequestration of Plasmodium berghei schizonts.PLoS pathogens · 2022Article
- Host cell maturation modulates parasite invasion and sexual differentiation inScience advances · 2022Article
- Chemoprophylaxis vaccination with a Plasmodium liver stage autophagy mutant affords enhanced and long-lasting protection.NPJ vaccines · 2021Article
- Differential Fractionation of Erythrocytes Infected byBio-protocol · 2020Article
- Gene knockdown in malaria parasites via non-canonical RNAi.Nucleic acids research · 2020Article
- Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development.Malaria journal · 2019Article
- Article
- Alternative splicing is required for stage differentiation in malaria parasites.Genome biology · 2019Article
- Improvement of an in vitro drug selection method for generating transgenic Plasmodium berghei parasites.Malaria journal · 2019Article
- Distinct Prominent Roles for Enzymes of Plasmodium berghei Heme Biosynthesis in Sporozoite and Liver Stage Maturation.Infection and immunity · 2016Article
- Comparative Plasmodium gene overexpression reveals distinct perturbation of sporozoite transmission by profilin.Molecular biology of the cell · 2016Article
- The subcellular location of ovalbumin in Plasmodium berghei blood stages influences the magnitude of T-cell responses.Infection and immunity · 2014Article
- A rapid and robust selection procedure for generating drug-selectable marker-free recombinant malaria parasites.Scientific reports · 2014Article
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Live cell imaging of recombinant malarial parasites encoding fluorescent probes provides critical insights into parasite-host interactions and life cycle progression. In this study, we generated a red fluorescent line of the murine malarial parasite Plasmodium berghei. To allow constitutive and abundant expression of the mCherry protein we profiled expression of all members of the P. berghei heat shock protein 70 (HSP70) family. We identified PbHSP70/1, an invariant ortholog of Plasmodium falciparum HSP70-1, as the protein with the highest expression levels during Plasmodium blood, mosquito, and liver infection. Stable allelic insertion of a mCherry expression cassette into the PbHsp70/1 locus created constitutive red fluorescent P. berghei lines, termed Pbred. We show that these parasites can be used for live imaging of infected host cells and organs, including hepatocytes, erythrocytes, and whole Anopheles mosquitoes. Quantification of the fluorescence intensity of several Pbred parasite stages revealed significantly enhanced signal intensities in comparison to GFP expressed under the control of the constitutive EF1alpha promoter. We propose that systematic transcript profiling permits generation of reporter parasites, such as the Pbred lines described herein.
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