ArticleBiomaterials2013
Functional ultrasound imaging for assessment of extracellular matrix scaffolds used for liver organoid formation.
Article in Biomaterials, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- [Liver engineering as a new source of donor organs : A systematic review].Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen · 2016Pooled it
- Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities.Bioactive materials · 2024Review
- Whole Liver Derived Acellular Extracellular Matrix for Bioengineering of Liver Constructs: An Updated Review.Bioengineering (Basel, Switzerland) · 2023Review
- Bioengineering Liver Organoids for Diseases Modelling and Transplantation.Bioengineering (Basel, Switzerland) · 2022Review
- ECM roles and biomechanics in cardiac tissue decellularization.American journal of physiology. Heart and circulatory physiology · 2022Review
- Use of 3-D Contrast-Enhanced Ultrasound to Evaluate Tumor Microvasculature After Nanoparticle-Mediated Modulation.Ultrasound in medicine & biology · 2020Article
- Calcified decellularized arterial scaffolds impact vascular smooth muscle cell transformation via downregulating α-SMA expression and upregulating OPN expression.Experimental and therapeutic medicine · 2019Article
- Advances in Hepatic Tissue Bioengineering with Decellularized Liver Bioscaffold.Stem cells international · 2019Review
- High-content imaging assays on a miniaturized 3D cell culture platform.Toxicology in vitro : an international journal published in association with BIBRA · 2018Article
- Acoustic Radiation Force Based Ultrasound Elasticity Imaging for Biomedical Applications.Sensors (Basel, Switzerland) · 2018Review
- Microfluidic-Based Multi-Organ Platforms for Drug Discovery.Micromachines · 2016Review
- Quantitative Ultrasound for Nondestructive Characterization of Engineered Tissues and Biomaterials.Annals of biomedical engineering · 2016Review
- Non-invasive and Non-destructive Characterization of Tissue Engineered Constructs Using Ultrasound Imaging Technologies: A Review.Annals of biomedical engineering · 2016Review
- Imaging strategies for tissue engineering applications.Tissue engineering. Part B, Reviews · 2015Review
- Reversible immortalization of human hepatocytes mediated by retroviral transfer and site-specific recombination.World journal of gastroenterology · 2014Article
- Decellularization for the retention of tissue niches.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
A method of 3D functional ultrasound imaging has been developed to enable non-destructive assessment of extracellular matrix scaffolds that have been prepared by decellularization protocols and are intended for recellularization to create organoids. A major challenge in organ decellularization is retaining patent micro-vascular structures crucial for nutrient access and functionality of organoids. The imaging method described here provides statistical distributions of flow rates throughout the tissue volumes, 3D vessel network architecture visualization, characterization of microvessel volumes and sizes, and delineation of matrix from vascular circuits. The imaging protocol was tested on matrix scaffolds that are tissue-specific, but not species-specific, matrix extracts, prepared by a process that preserved >98% of the collagens, collagen-associated matrix components, and matrix-bound growth factors and cytokines. Image-derived data are discussed with respect to assessment of scaffolds followed by proof-of-concept studies in organoid establishment using Hep3B, a human hepatoblast-like cell line. Histology showed that the cells attached to scaffolds with patent vasculature within minutes, achieved engraftment at near 100%, expressed liver-specific functions within 24 h, and yielded evidence of proliferation and increasing differentiation of cells throughout the two weeks of culture studies. This imaging method should prove valuable in analyses of such matrix scaffolds.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.