ArticleBMC plant biology2013
Molecular cloning and characterisation of SlAGO family in tomato.
Article in BMC plant biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 42 citations in OpenAlex.
- Resistance gene Ty-1 restricts TYLCV infection in tomato by increasing RNA silencing.Virology journal · 2024Article
- Function and regulation of plant ARGONAUTE proteins in response to environmental challenges: a review.PeerJ · 2024Review
- Tomato MicroRNAs and Their Functions.International journal of molecular sciences · 2022Review
- RNAi-Based Antiviral Innate Immunity in Plants.Viruses · 2022Review
- Tomato salt tolerance mechanisms and their potential applications for fighting salinity: A review.Frontiers in plant science · 2022Review
- Genome-wide identification of Argonautes in Solanaceae with emphasis on potato.Scientific reports · 2020Article
- miR168 targets Argonaute1A mediated miRNAs regulation pathways in response to potassium deficiency stress in tomato.BMC plant biology · 2020Article
- New insights into tomato microRNAs.Scientific reports · 2018Article
- Generation of an integrated Hieracium genomic and transcriptomic resource enables exploration of small RNA pathways during apomixis initiation.BMC biology · 2016Article
- Identification and functional analysis of novel and conserved microRNAs in tomato.Molecular biology reports · 2014Article
- Suppress to Survive-Implication of Plant Viruses in PTGS.Plant molecular biology reporterReview
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAGO (Argonaute) protein participates in plant developmental processes and virus defense as a core element of transcriptional regulator or/and post-transcriptional regulator in RNA induced silencing complex (RISC), which is guided by small RNAs to repress target genes expression. Previously, it was revealed that 15 putative AGO genes in tomato genome.
resultsIn present study, out of 15 detected SlAGO genes, only SlAGO4C and SlAGO15 couldn't be detected in roots, stems, leaves, buds, flowers and fruit of tomato by 30 cycles of PCR. SlAGO7 could be detected in early stage of fruit (-2 dpa, 0 dpa and 4 dpa), but it was significantly down-regulated in fruit collected on the 6 days post anthesis. Moreover, SlAGO5 could only be detected in reproductive tissues and SlAGO4D was specifically detected in fruit. According to blast result with miRNA database, three SlAGO genes harbored complementary sequences to miR168 (SlAGO1A and SlAGO1B) or miR403 (SlAGO2A). 5' RACE (Rapid amplification of cDNA ends) mapping was used to detect the 3' cleavage products of SlAGO mRNAs. In addition, subcellular localization of SlAGO proteins was detected. Our results showed that most SlAGO proteins localized to nucleus and cytoplasm. Importantly, nuclear membrane localization of AGO proteins was observed. Furthermore, mutated miR168 complementary site of SlAGO1A resulted in expanded localization of SlAGO1A, indicating that miR168 regulated localization of SlAGO1A.
conclusionsOur results contribute to demonstration of potential roles of these newly isolated AGO family in tomato developmental processes and proved the conserved relationships between AGO genes and miRNAs in tomato, which might play important roles in tomato development and virus defense.
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