ArticleAmerican journal of respiratory cell and molecular biology2014
Directional secretomes reflect polarity-specific functions in an in vitro model of human bronchial epithelium.
Article in American journal of respiratory cell and molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Desmoglein-2 dysregulation impairs epithelial barrier integrity in eosinophilic asthma.Respiratory research · 2026Article
- Fungal melanin suppresses airway epithelial chemokine secretion through blockade of calcium fluxing.Nature communications · 2024Article
- EPsInternational journal of molecular sciences · 2023Article
- Lung epithelial cell-derived C3 protects against pneumonia-induced lung injury.Science immunology · 2023Article
- Genes, environment, and developmental timing: New insights from translational approaches to understand early origins of respiratory diseases.Pediatric pulmonology · 2021Article
- The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins.Molecular & cellular proteomics : MCP · 2020Article
- Apolipoprotein E and Periostin Are Potential Biomarkers of Nasal Mucosal Inflammation. A Parallel Approach ofAmerican journal of respiratory cell and molecular biology · 2020Article
- Oxidative stress-induced inflammation in susceptible airways by anthropogenic aerosol.PloS one · 2020Article
- Intracellular C3 Protects Human Airway Epithelial Cells from Stress-associated Cell Death.American journal of respiratory cell and molecular biology · 2019Article
- The complement system in the airway epithelium: An overlooked host defense mechanism and therapeutic target?The Journal of allergy and clinical immunology · 2018Review
- Differential regulation of the transcriptomic and secretomic landscape of sensor and effector functions of human airway epithelial cells.Mucosal immunology · 2018Article
- New Strategies and Challenges in Lung Proteomics and Metabolomics. An Official American Thoracic Society Workshop Report.Annals of the American Thoracic Society · 2017Article
- The Diacetyl-Exposed Human Airway Epithelial Secretome: New Insights into Flavoring-Induced Airways Disease.American journal of respiratory cell and molecular biology · 2017Article
- An in vitro model of murine middle ear epithelium.Disease models & mechanisms · 2016Article
- Article
- Rhinovirus-Induced Airway Disease: A Model to Understand the Antiviral and Th2 Epithelial Immune Dysregulation in Childhood Asthma.Journal of investigative medicine : the official publication of the American Federation for Clinical Research · 2015Review
- Quantitative proteomics reveals an altered cystic fibrosis in vitro bronchial epithelial secretome.American journal of respiratory cell and molecular biology · 2015Article
- Article
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8 authors at 2 institutions in 1 country.
Funding
Abstract
The polarity of the conducting airway epithelium is responsible for its directional secretion. This is an essential characteristic of lung integrity and function that dictates interactions between the external environment (apical) and subepithelial structures (basolateral). Defining the directional secretomes in the in vitro human bronchial epithelial (HBE) differentiated model could bring valuable insights into lung biology and pulmonary diseases. Normal primary HBE cells (n = 3) were differentiated into respiratory tract epithelium. Apical and basolateral secretions (24 h) were processed for proteome profiling and pathway analysis. A total of 243 proteins were identified in secretions from all HBE cultures combined. Of these, 51% were classified as secreted proteins, including true secreted proteins (36%) and exosomal proteins (15%). Close examination revealed consistent secretion of 69 apical proteins and 13 basolateral proteins and differential secretion of 25 proteins across all donors. Expression of Annexin A4 in apical secretions and Desmoglein-2 in basolateral secretions was validated using Western blot or ELISA in triplicate independent experiments. To the best of our knowledge, this is the first study defining apical and basolateral secretomes in the in vitro differentiated HBE model. The data demonstrate that epithelial polarity directs protein secretion with different patterns of biological processes to the apical and basolateral surfaces that are consistent with normal bronchial epithelium homeostatic functions. Applying this in vitro directional secretome model to lung diseases may elucidate their molecular pathophysiology and help define potential therapeutic targets.
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