ArticleAnalytical chemistry2013
An integrated microfluidic device for monitoring changes in nitric oxide production in single T-lymphocyte (Jurkat) cells.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- The Bright and Dark Sides of Nitric Oxide in Neurodegenerative Diseases.Journal of personalized medicine · 2026Review
- Microfluidic single-cell measurements of oxidative stress as a function of cell cycle position.Analytical and bioanalytical chemistry · 2023Article
- Prenatal stress induces a depressive-like phenotype in adolescent rats: The key role of TGF-β1 pathway.Frontiers in pharmacology · 2022Article
- Harnessing Joule heating in microfluidic thermal gel electrophoresis to create reversible barriers for cell enrichment.Electrophoresis · 2021Article
- Antioxidant Activity of Fluoxetine and Vortioxetine in a Non-Transgenic Animal Model of Alzheimer's Disease.Frontiers in pharmacology · 2021Article
- Microfluidics as a Novel Tool for Biological and Toxicological Assays in Drug Discovery Processes: Focus on Microchip Electrophoresis.Micromachines · 2020Review
- Carnosine Decreases PMA-Induced Oxidative Stress and Inflammation in Murine Macrophages.Antioxidants (Basel, Switzerland) · 2019Article
- Carnosine Prevents Aβ-Induced Oxidative Stress and Inflammation in Microglial Cells: A Key Role of TGF-β1.Cells · 2019Article
- Optimization of a microchip electrophoresis method with electrochemical detection for the determination of nitrite in macrophage cells as an indicator of nitric oxide production.Analytical methods : advancing methods and applications · 2019Article
- Carnosine modulates nitric oxide in stimulated murine RAW 264.7 macrophages.Molecular and cellular biochemistry · 2017Article
- 3D-printed Microfluidic Devices: Fabrication, Advantages and Limitations-a Mini Review.Analytical methods : advancing methods and applications · 2016Article
- Get to Understand More from Single-Cells: Current Studies of Microfluidic-Based Techniques for Single-Cell Analysis.International journal of molecular sciences · 2015Review
- Integrated hybrid polystyrene-polydimethylsiloxane device for monitoring cellular release with microchip electrophoresis and electrochemical detection.Analytical methods : advancing methods and applications · 2015Article
- Microchip electrophoresis with amperometric detection method for profiling cellular nitrosative stress markers.The Analyst · 2014Article
- Response of single leukemic cells to peptidase inhibitor therapy across time and dose using a microfluidic device.Integrative biology : quantitative biosciences from nano to macro · 2014Article
- Nitric oxide detection methodsMedical gas researchReview
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
A considerable amount of attention has been focused on the analysis of single cells in an effort to better understand cell heterogeneity in cancer and neurodegenerative diseases. Although microfluidic devices have several advantages for single cell analysis, few papers have actually demonstrated the ability of these devices to monitor chemical changes in perturbed biological systems. In this paper, a new microfluidic channel manifold is described that integrates cell transport, lysis, injection, electrophoretic separation, and fluorescence detection into a single device, making it possible to analyze individual cells at a rate of 10 cells/min in an automated fashion. The system was employed to measure nitric oxide (NO) production in single T-lymphocytes (Jurkat cells) using a fluorescent marker, 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate (DAF-FM DA). The cells were also labeled with 6-carboxyfluorescein diacetate (6-CFDA) as an internal standard. The NO production by control cells was compared to that of cells stimulated using lipopolysaccharide (LPS), which is known to cause the expression of inducible nitric oxide synthase (iNOS) in immune-type cells. Statistical analysis of the resulting electropherograms from a population of cells indicated a 2-fold increase in NO production in the induced cells. These results compare nicely to a recently published bulk cell analysis of NO.
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Registered trials
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