ArticleBMC complementary and alternative medicine2013
The effect of Shenmai injection on the proliferation of Rat airway smooth muscle cells in asthma and underlying mechanism.
Article in BMC complementary and alternative medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Serum from acupuncture-treated asthmatic rats regulates p38-MAPK activation in airway smooth muscle cells.American journal of translational research · 2023Article
- A narrative review of research advances in the study of molecular markers of airway smooth muscle cells.Annals of translational medicine · 2022Review
- Chloroquine Attenuates Asthma Development by Restoring Airway Smooth Muscle Cell PhenotypeFrontiers in pharmacology · 2022Article
- Investigation of the Active Ingredients and Mechanism ofDrug design, development and therapy · 2021Article
- The Inhibition Effects of Shenmai Injection on Acetylcholine-Induced Catecholamine Synthesis and Secretion by Modulating Nicotinic Acetylcholine Receptor Ion Channels in Cultured Bovine Adrenal Medullary Cells.Evidence-based complementary and alternative medicine : eCAM · 2020Article
- P2X4R promotes airway remodeling by acting on the phenotype switching of bronchial smooth muscle cells in rats.Purinergic signalling · 2018Article
- Protective effect of Shenmai injection on knee articular cartilage of osteoarthritic rabbits and IL-1β-stimulated human chondrocytes.Experimental and therapeutic medicine · 2017Article
- Ophiopogonin D maintains Ca2+ homeostasis in rat cardiomyocytes in vitro by upregulating CYP2J3/EETs and suppressing ER stress.Acta pharmacologica Sinica · 2016Article
- Effect of P2X4R on airway inflammation and airway remodeling in allergic airway challenge in mice.Molecular medicine reports · 2016Article
- Neural Abnormalities in Nonallergic Rhinitis.Current allergy and asthma reports · 2015Review
- A viral vector expressing hypoxia-inducible factor 1 alpha inhibits hippocampal neuronal apoptosis.Neural regeneration research · 2014Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOver-proliferation of airway smooth muscle cell (ASMC) is one of the important contributors to airway remodeling in asthma. The aim of this study was to investigate the effect of Shenmai injection (SMI) on the proliferation of the rat ASMC in asthma.
methodsRats were randomly divided into three groups: the control group, the asthma group, and the SMI treatment group. Reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry staining were used to detect the mRNA and protein expression of transient receptor potential vanilloid 1 (TRPV1) and proliferating cell nuclear antigen (PCNA) in rat ASMC respectively. Intracellular Ca²⁺ concentration ( [Ca²⁺](i)) in rat ASMC were measured with Fluo-3/AM by confocal microscopy. The proliferation was detected by MTT assay.
resultsCompared with the control group, the asthma group showed an increased expression of TRPV1 and [Ca²⁺](i) in rat ASMC. The expression of PCNA and absorbance of MTT assay in asthma rat ASMC was also significantly increased. SMI could significantly decrease the expression of TRPV1 channel and [Ca²⁺](i) in the asthmatic rat ASMC. Furthermore, the expression of PCNA and absorbance of MTT assay in asthmatic rat ASMC was significantly reduced after SMI treatment.
conclusionsSMI may prevent asthma-induced ASMC over-proliferation probably by inhibiting the expression of TRPV1 channel, which regulates the intracellular calcium concentration.
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