ArticleBiophysical journal2013
Superresolution imaging of dynamic MreB filaments in B. subtilis--a multiple-motor-driven transport?
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Localization and Single Molecule Dynamics ofCells · 2025Article
- Adaptation ofMicroorganisms · 2024Article
- Molecular motor tug-of-war regulates elongasome cell wall synthesis dynamics in Bacillus subtilis.Nature communications · 2024Article
- Article
- Models versus pathogens: how conserved is the FtsZ in bacteria?Bioscience reports · 2023Review
- Through the Eyes of Creators: Observing Artificial Molecular Motors.ACS nanoscience Au · 2022Review
- Self-association of MreC as a regulatory signal in bacterial cell wall elongation.Nature communications · 2021Article
- The role of the cytoskeletal proteins MreB and FtsZ in multicellular cyanobacteria.FEBS open bio · 2020Article
- Polymerization of Bacillus subtilis MreB on a lipid membrane reveals lateral co-polymerization of MreB paralogs and strong effects of cations on filament formation.BMC molecular and cell biology · 2020Article
- Regulation of peptidoglycan synthesis and remodelling.Nature reviews. Microbiology · 2020Review
- Fast TIRF-SIM imaging of dynamic, low-fluorescent biological samples.Biomedical optics express · 2020Article
- Article
- Super-Resolution Microscopy and Single-Molecule Tracking Reveal Distinct Adaptive Dynamics of MreB and of Cell Wall-Synthesis Enzymes.Frontiers in microbiology · 2020Article
- The evolution of spherical cell shape; progress and perspective.Biochemical Society transactions · 2019Review
- Bacillus subtilis cell diameter is determined by the opposing actions of two distinct cell wall synthetic systems.Nature microbiology · 2019Article
- Visualization of Bacterial Protein Complexes Labeled with Fluorescent Proteins and Nanobody Binders for STED Microscopy.International journal of molecular sciences · 2019Article
- Article
- A specialized MreB-dependent cell wall biosynthetic complex mediates the formation of stalk-specific peptidoglycan in Caulobacter crescentus.PLoS genetics · 2019Article
- Article
- Dynamics of a Protein Chain Motor Driving Helical Bacteria under Stress.Biophysical journal · 2018Article
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6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cytoskeletal protein MreB is an essential component of the bacterial cell-shape generation system. Using a superresolution variant of total internal reflection microscopy with structured illumination, as well as three-dimensional stacks of deconvolved epifluorescence microscopy, we found that inside living Bacillus subtilis cells, MreB forms filamentous structures of variable lengths, typically not longer than 1 μm. These filaments move along their orientation and mainly perpendicular to the long bacterial axis, revealing a maximal velocity at an intermediate length and a decreasing velocity with increasing filament length. Filaments move along straight trajectories but can reverse or alter their direction of propagation. Based on our measurements, we provide a mechanistic model that is consistent with all observations. In this model, MreB filaments mechanically couple several motors that putatively synthesize the cell wall, whereas the filaments' traces mirror the trajectories of the motors. On the basis of our mechanistic model, we developed a mathematical model that can explain the nonlinear velocity length dependence. We deduce that the coupling of cell wall synthesis motors determines the MreB filament transport velocity, and the filament mechanically controls a concerted synthesis of parallel peptidoglycan strands to improve cell wall stability.
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