ArticleSpringerPlus2013
Novel xylanases from Simplicillium obclavatum MTCC 9604: comparative analysis of production, purification and characterization of enzyme from submerged and solid state fermentation.
Article in SpringerPlus, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Recent advances of fermented fruits: A review on strains, fermentation strategies, and functional activities.Food chemistry: X · 2024Review
- A review on xylanase sources, classification, mode of action, fermentation processes, and applications as a promising biocatalyst.Biotechnologia · 2024Review
- Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean HullsFood technology and biotechnology · 2023Article
- Article
- Optimization, purification, and characterization of xylanase production by a newly isolated Trichoderma harzianum strain by a two-step statistical experimental design strategy.Scientific reports · 2022Article
- Valorization of Brewer's Spent Grain Using Biological Treatments and its Application in Feeds for European Seabass (Frontiers in bioengineering and biotechnology · 2022Article
- Solid-state fermented brewer's spent grain enzymatic extract increases in vitro and in vivo feed digestibility in European seabass.Scientific reports · 2021Article
- Simplicilones A and B Isolated from the Endophytic FungusAntibiotics (Basel, Switzerland) · 2020Article
- High-Level Heterologous Expression of Endo-1,4-β-Xylanase fromMolecules (Basel, Switzerland) · 2019Article
- Three novel insect-associated species ofMycoKeys · 2019Article
- The genusMycoKeys · 2019Article
- Production, Purification, and Characterization of Thermostable Alkaline Xylanase FromFrontiers in bioengineering and biotechnology · 2018Article
- Bacterial xylanases: biology to biotechnology.3 Biotech · 2016Review
- (1→3)-β-d-Glucan and Galactomannan for Differentiating Chemical "Black Particles" and Fungal Particles Inside Peritoneal Dialysis Tubing.Peritoneal dialysis international : journal of the International Society for Peritoneal DialysisArticle
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Authors and funding
4 authors.
Funding
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Abstract
The production of extracellular xylanase by a newly isolated fungus Simplicillium obclavatum MTCC 9604 was studied in solid-state and submerged fermentation. Multiple xylanases and endoglucanases were produced by the strain during growth on wheat bran in solid state fermentation (SSF). A single xylanase isoform was found to be produced by the same fungus under submerged fermentation (SF) using wheat bran as sole carbon source. Enzyme activity, stability and the protein yield were much higher in SSF than SF. The two dimensional zymogram of the crude enzyme indicated the presence of six isoforms with different pI values starting from pH 3-10. The optimum temperature and pH for the partially purified xylanase activity were 50°C and pH 5.0 respectively; xylanase enzymes exhibited remarkable stability over a broad pH range and the temperature range of 30-60°C which has great potential to be used in biofuels, animal feed and food industry applications.
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