ArticleBiomedical optics express2013
Subcellular localization-dependent changes in EGFP fluorescence lifetime measured by time-resolved flow cytometry.
Article in Biomedical optics express, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Fluorescence Lifetime Measurements and Analyses: Protocols Using Flow Cytometry and High-Throughput Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues.Frontiers in physics · 2021Article
- Lifetime encoding in flow cytometry for bead-based sensing of biomolecular interaction.Scientific reports · 2020Article
- Enrichment of rare events using a multi-parameter high throughput microfluidic droplet sorter.Lab on a chip · 2020Article
- Tempo-spectral multiplexing in flow cytometry with lifetime detection using QD-encoded polymer beads.Scientific reports · 2020Article
- Multiphoton FLIM imaging of NAD(P)H and FAD with one excitation wavelength.Journal of biomedical optics · 2020Article
- Luminescence lifetime encoding in time-domain flow cytometry.Scientific reports · 2018Article
- Subcellular Peptide Localization in Single Identified Neurons by Capillary Microsampling Mass Spectrometry.Scientific reports · 2018Article
- Analysis of Flow Cytometric Fluorescence Lifetime with Time-Delay Estimation of Pulse Signals.Sensors (Basel, Switzerland) · 2018Article
- Metabolic Dependencies in Pancreatic Cancer.Frontiers in oncology · 2018Review
- Shifts in the fluorescence lifetime of EGFP during bacterial phagocytosis measured by phase-sensitive flow cytometry.Scientific reports · 2017Article
- Representation Method for Spectrally Overlapping Signals in Flow Cytometry Based on Fluorescence Pulse Time-Delay Estimation.Sensors (Basel, Switzerland) · 2016Article
- Phasor plotting with frequency-domain flow cytometry.Optics express · 2016Article
- Toward the measurement of multiple fluorescence lifetimes in flow cytometry: maximizing multi-harmonic content from cells and microspheres.Journal of biophotonics · 2015Article
- Time-domain microfluidic fluorescence lifetime flow cytometry for high-throughput Förster resonance energy transfer screening.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2015Article
- Expanding the potential of standard flow cytometry by extracting fluorescence lifetimes from cytometric pulse shifts.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2014Article
- Fluorescence lifetime excitation cytometry by kinetic dithering.Electrophoresis · 2014Article
- Measuring and sorting cell populations expressing isospectral fluorescent proteins with different fluorescence lifetimes.PloS one · 2014Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Intracellular protein transport and localization to subcellular regions are processes necessary for normal protein function. Fluorescent proteins can be fused to proteins of interest to track movement and determine localization within a cell. Currently, fluorescence microscopy combined with image processing is most often used to study protein movement and subcellular localization. In this contribution we evaluate a high-throughput time-resolved flow cytometry approach to correlate intracellular localization of human LC3 protein with the fluorescence lifetime of enhanced green fluorescent protein (EGFP). Subcellular LC3 localization to autophagosomes is a marker of the cellular process called autophagy. In breast cancer cells expressing native EGFP and EGFP-LC3 fusion proteins, we measured the fluorescence intensity and lifetime of (i) diffuse EGFP (ii) punctate EGFP-LC3 and (iii) diffuse EGFP-ΔLC3 after amino acid starvation to induce autophagy-dependent LC3 localization. We verify EGFP-LC3 localization with low-throughput confocal microscopy and compare to fluorescence intensity measured by standard flow cytometry. Our results demonstrate that time-resolved flow cytometry can be correlated to subcellular localization of EGFP fusion proteins by measuring changes in fluorescence lifetime.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.