ArticleInvestigative ophthalmology & visual science2013
CAP37 activation of PKC promotes human corneal epithelial cell chemotaxis.
Article in Investigative ophthalmology & visual science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Antimicrobial Peptide Expression at the Ocular Surface and Their Therapeutic Use in the Treatment of Microbial Keratitis.Frontiers in microbiology · 2022Review
- Antimicrobial Peptides Derived from the Immune Defense Protein CAP37 Inhibit TLR4 Activation by S100A9.Investigative ophthalmology & visual science · 2020Article
- A Promising Candidate: Heparin-Binding Protein Steps onto the Stage of Sepsis Prediction.Journal of immunology research · 2019Review
- The role of neutrophil granule proteins in neuroinflammation and Alzheimer's disease.Journal of neuroinflammation · 2018Review
- Bioactive Antimicrobial Peptides as Therapeutics for Corneal Wounds and Infections.Advances in wound care · 2017Review
- Effect of Cationic Antimicrobial Protein CAP37 on Cytokine Profile during Corneal Wound Healing.Journal of ocular diseases and therapeutics · 2017Article
- Heparin-binding protein is important for vascular leak in sepsis.Intensive care medicine experimental · 2016Article
- The Histochemistry and Cell Biology omnium-gatherum: the year 2015 in review.Histochemistry and cell biology · 2016Review
- The antimicrobial protein, CAP37, is upregulated in pyramidal neurons during Alzheimer's disease.Histochemistry and cell biology · 2015Article
- A multifunctional peptide based on the neutrophil immune defense molecule, CAP37, has antibacterial and wound-healing properties.Journal of leukocyte biology · 2015Article
- Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.International journal of ophthalmology · 2015Article
- Corneal wound healing, a newly identified function of CAP37, is mediated by protein kinase C delta (PKCδ).Investigative ophthalmology & visual science · 2014Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe objective of this study was to elucidate the signaling pathway through which cationic antimicrobial protein of 37 kDa (CAP37) mediates human corneal epithelial cell (HCEC) chemotaxis.
methodsImmortalized HCECs were treated with pertussis toxin (10 and 1000 ng/mL), protein kinase C (PKC) inhibitors (calphostin c, 50 nM and Ro-31-8220, 100 nM), phorbol esters (phorbol 12,13-dibutyrate, 200 nM and phorbol 12-myristate 13-acetate, 1 μM) known to deplete PKC isoforms, and siRNAs (400 nM) before a modified Boyden chamber assay was used to determine the effect of these inhibitors and siRNAs on CAP37-directed HCEC migration. PKCδ protein levels, PKCδ-Thr(505) phosphorylation, and PKCδ kinase activity was assessed in CAP37-treated HCECs using immunohistochemistry, Western blotting, and a kinase activity assay, respectively.
resultsChemotaxis studies revealed that treatment with pertussis toxin, PKC inhibitors, phorbol esters, and siRNAs significantly inhibited CAP37-mediated chemotaxis compared with untreated controls. CAP37 treatment increased PKCδ protein levels and led to PKCδ phosphorylation on residue Thr(505). Direct activation of PKCδ by CAP37 was demonstrated using a kinase activity assay.
conclusionsThese findings lead us to conclude that CAP37 is an important regulator of corneal epithelial cell migration and mediates its effects through PKCδ.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.