ArticleNature protocols2013
Generation of T cell receptor-retrogenic mice: improved retroviral-mediated stem cell gene transfer.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 40 citations in OpenAlex.
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- Identification of Sjögren's disease-associated T cell receptor motifs through deep sequencing.JCI insight · 2025Article
- Human MAIT cells undergo clonal selection and expansion during thymic maturation and aging.Experimental & molecular medicine · 2025Article
- T-Cell Receptor/HLA Humanized Mice Reveal Reduced Tolerance and Increased Immunogenicity of Posttranslationally Modified GAD65 Epitope.Diabetes · 2022Article
- Nanoscale Colocalization of NK Cell Activating and Inhibitory Receptors Controls Signal Integration.Frontiers in immunology · 2022Article
- Metabolic control of TNature · 2021Article
- Beryllium-specific CD4+ T cells induced by chemokine neoantigens perpetuate inflammation.The Journal of clinical investigation · 2021Article
- Proinsulin-Reactive CD4 T Cells in the Islets of Type 1 Diabetes Organ Donors.Frontiers in endocrinology · 2021Article
- Egr2 and 3 control inflammation, but maintain homeostasis, of PD-1Life science alliance · 2020Article
- A Critical Insulin TCR Contact Residue Selects High-Affinity and Pathogenic Insulin-Specific T Cells.Diabetes · 2020Article
- A Hybrid Insulin Epitope Maintains High 2D Affinity for Diabetogenic T Cells in the Periphery.Diabetes · 2020Article
- Multiplex T Cell Stimulation Assay Utilizing a T Cell Activation Reporter-Based Detection System.Frontiers in immunology · 2020Article
- A distinct subset of FcγRI-expressing Th1 cells exert antibody-mediated cytotoxic activity.The Journal of clinical investigation · 2019Article
- Generation of T Cell Receptor Retrogenic Mice.Current protocols in immunology · 2019Article
- Chimeric antigen receptor (CAR) T cells targeting a pathogenic MHC class II:peptide complex modulate the progression of autoimmune diabetes.Journal of autoimmunity · 2019Article
- Development of T cell lines sensitive to antigen stimulation.Journal of immunological methods · 2018Article
- Mycobacterium tuberculosis-specific CD4+ and CD8+ T cells differ in their capacity to recognize infected macrophages.PLoS pathogens · 2018Article
- Article
- Expanding the Toolkit for the Study of Allospecific B and T Cell Responses.Transplantation · 2017Article
- Ectopic Expression of Self-Antigen Drives Regulatory T Cell Development and Not Deletion of Autoimmune T Cells.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The use of retrogenic mice offers a rapid and flexible approach to T cell receptor (TCR)-transgenic mice. By transducing bone marrow progenitor cells with a retrovirus that encodes a given TCR-α/β subunit, TCR-retrogenic mice can be generated in as few as 4-6 weeks, whereas conventional TCR transgenics can take 6 months or longer. In this updated protocol, we have increased the efficiency of the bone marrow transduction and bone marrow reconstitution compared with our previously published protocol. The main departure from the previous protocol is the implementation of spin transduction with the viral supernatant instead of coculture with the viral producer cell line. The changes in this protocol improve bone marrow viability, increase consistency of the bone marrow transduction and bone marrow engraftment, and they reduce the ratio of bone marrow donor mice to bone marrow recipients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.