ArticleTissue engineering. Part C, Methods2014
Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 1: investigation of cocultures and seeding densities for improved extracellular matrix deposition.
Article in Tissue engineering. Part C, Methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Co-culture pellet of human Wharton's jelly mesenchymal stem cells and rat costal chondrocytes as a candidate for articular cartilage regeneration: in vitro and in vivo study.Stem cell research & therapy · 2022Article
- [Research progress of different cell seeding densities and cell ratios in cartilage tissue engineering].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2022Review
- Crosstalk Between Mesenchymal Stromal Cells and Chondrocytes: The Hidden Therapeutic Potential for Cartilage Regeneration.Stem cell reviews and reports · 2021Review
- Chondrogenesis of cocultures of mesenchymal stem cells and articular chondrocytes in poly(l-lysine)-loaded hydrogels.Journal of controlled release : official journal of the Controlled Release Society · 2020Article
- Extracellular Matrix for Small-Diameter Vascular Grafts.Tissue engineering. Part A · 2020Review
- PLGA scaffold carrying icariin to inhibit the progression of osteoarthritis in rabbits.Royal Society open science · 2019Article
- Functionality of decellularized matrix in cartilage regeneration: A comparison of tissue versus cell sources.Acta biomaterialia · 2018Review
- Combating Osteoarthritis through Stem Cell Therapies by Rejuvenating Cartilage: A Review.Stem cells international · 2018Review
- Cell type-specific extracellular matrix guided the differentiation of human mesenchymal stem cells in 3D polymeric scaffolds.Journal of materials science. Materials in medicine · 2017Article
- Optimization of Polymer-ECM Composite Scaffolds for Tissue Engineering: Effect of Cells and Culture Conditions on Polymeric Nanofiber Mats.Journal of functional biomaterials · 2017Article
- Concise review: tailoring bioengineered scaffolds for stem cell applications in tissue engineering and regenerative medicine.Stem cells translational medicine · 2015Review
- Biomimetic extracellular matrix mediated somatic stem cell differentiation: applications in dental pulp tissue regeneration.Frontiers in physiology · 2015Review
- Enhancing chondrogenic phenotype for cartilage tissue engineering: monoculture and coculture of articular chondrocytes and mesenchymal stem cells.Tissue engineering. Part B, Reviews · 2014Review
- Multifunctional ECM proteins in bone and teeth.Experimental cell research · 2014Review
- Direct and indirect co-culture of chondrocytes and mesenchymal stem cells for the generation of polymer/extracellular matrix hybrid constructs.Acta biomaterialia · 2014Article
- Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.Tissue engineering. Part C, Methods · 2014Article
- Stem cells catalyze cartilage formation by neonatal articular chondrocytes in 3D biomimetic hydrogels.Scientific reports · 2013Article
- Post-decellularization techniques ameliorate cartilage decellularization process for tissue engineering applications.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
This study investigated the coculture of chondrocytes and mesenchymal stem cells (MSCs) on electrospun fibrous polymer scaffolds to produce polymer/extracellular matrix (ECM) hybrid constructs with the objective of reducing the number of chondrocytes necessary to produce ample cartilage-like ECM within the scaffolds. To generate these hybrid constructs, electrospun poly(ɛ-caprolactone) fibrous scaffolds were seeded at both high and low initial densities with five different ratios of chondrocytes to MSCs: 1:0, 1:1, 1:3, 1:5, and 0:1, and cultured for 7, 14, and 21 days. Glycosaminoglycan production and distribution within the three coculture groups was similar to quantities generated by chondrocyte-only controls. Conversely, as the concentration of chondrocytes was increased, the collagen content of the constructs also increased at each time point, with a 1:1 chondrocyte to MSC ratio approximating the collagen production of chondrocytes alone. Histological staining suggested that cocultured constructs mimicked the well-distributed ECM patterns of chondrocyte generated constructs, while improving greatly over the restricted distribution of matrix within MSC-only constructs. These results support the capacity of cocultures of chondrocytes and MSCs to generate cartilaginous matrix within a polymeric scaffold. Further, the inclusion of MSCs in these cocultures enables the reduction of chondrocytes needed to produce cell-generated ECM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.