ArticleBMC microbiology2013
Quantitative analysis of the Brucella suis proteome reveals metabolic adaptation to long-term nutrient starvation.
Article in BMC microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
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- Proteomics of Brucella.Proteomes · 2020Review
- Proteomic Analysis of Stationary Growth Stage Adaptation and Nutritional Deficiency Response ofFrontiers in microbiology · 2020Article
- Extreme slow growth as alternative strategy to survive deep starvation in bacteria.Nature communications · 2019Article
- Proteogenomics in Aid of Host-Pathogen Interaction Studies: A Bacterial Perspective.Proteomes · 2017Review
- RegA Plays a Key Role in Oxygen-Dependent Establishment of Persistence and in Isocitrate Lyase Activity, a Critical Determinant ofFrontiers in cellular and infection microbiology · 2017Article
- Relative Quantitative Proteomic Analysis ofFrontiers in microbiology · 2017Article
- The Bacterial Second Messenger Cyclic di-GMP Regulates Brucella Pathogenesis and Leads to Altered Host Immune Response.Infection and immunity · 2016Article
- Proteomic analysis of Chromobacterium violaceum and its adaptability to stress.BMC microbiology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDuring the infection process, bacteria are confronted with various stress factors including nutrient starvation. In an in vitro model, adaptation strategies of nutrient-starved brucellae, which are facultative intracellular pathogens capable of long-term persistence, were determined.
resultsLong-term nutrient starvation in a medium devoid of carbon and nitrogen sources resulted in a rapid decline in viability of Brucella suis during the first three weeks, followed by stabilization of the number of viable bacteria for a period of at least three weeks thereafter. A 2D-Difference Gel Electrophoresis (DIGE) approach allowed the characterization of the bacterial proteome under these conditions. A total of 30 proteins showing altered concentrations in comparison with bacteria grown to early stationary phase in rich medium were identified. More than half of the 27 significantly regulated proteins were involved in bacterial metabolism with a marked reduction of the concentrations of enzymes participating in amino acid and nucleic acid biosynthesis. A total of 70% of the significantly regulated proteins showed an increased expression, including proteins involved in the adaptation to harsh conditions, in regulation, and in transport.
conclusionsThe adaptive response of Brucella suis most likely contributes to the long-term survival of the pathogen under starvation conditions, and may play a key role in persistence.
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