ArticleJournal of experimental botany2013
A novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigments.
Article in Journal of experimental botany, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 215 citations in OpenAlex.
- AlleleMiner: a long-read pipeline for gene-wise de novo allele phasing and variant detection in diploid citrus cultivars.DNA research : an international journal for rapid publication of reports on genes and genomes · 2026Article
- Targeted Gene Modification ofInternational journal of molecular sciences · 2026Article
- Regulation of apocarotenoids for quality improvement and biofortification of horticultural crops.Journal of advanced research · 2026Review
- An evolutionarily diverged CCD4 enzyme negatively regulates mesocotyl elongation in rice.The New phytologist · 2026Article
- Evolutionary adaptation of CCD4 enzymes inFrontiers in plant science · 2026Article
- LYCOPENE β-CYCLASE overexpression improves growth, modulates hormone content, and affects rhizospheric interactions in tobacco and tomato roots.Plant cell reports · 2025Article
- Ripening-Related Changes in Color and Bioactive Compounds ofFoods (Basel, Switzerland) · 2025Article
- Red peel regulator 1 links ethylene response factor 25 and β-citraurin biosynthetic genes to regulate ethylene-induced peel reddening in citrus.The Plant cell · 2024Article
- Exogenous application of the apocarotenoid retinaldehyde negatively regulates auxin-mediated root growth.Plant physiology · 2024Article
- Transcriptional deciphering of the metabolic pathways associated with the bioactive ingredients of wolfberry species with different quality characteristics.BMC genomics · 2023Article
- Measuring the Residual Levels of Fenpyroximate and Its Z-Isomer in Citrus Using Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry and Assessing the Related Dietary Intake Risks.Molecules (Basel, Switzerland) · 2023Article
- Antioxidant Interactions between Citrus Fruit Carotenoids and Ascorbic Acid in New Models of Animal Cell Membranes.Antioxidants (Basel, Switzerland) · 2023Article
- Natural overexpression of CAROTENOID CLEAVAGE DIOXYGENASE 4 in tomato alters carotenoid flux.Plant physiology · 2023Article
- Insights into the Genes Involved in ABA Biosynthesis and Perception during Development and Ripening of the Chilean Strawberry Fruit.International journal of molecular sciences · 2023Article
- Integrated metabolic and transcriptional analysis reveals the role of carotenoid cleavage dioxygenase 4 (IbCCD4) in carotenoid accumulation in sweetpotato tuberous roots.Biotechnology for biofuels and bioproducts · 2023Article
- Analysis of apocarotenoid volatiles from lettuce (3 Biotech · 2023Article
- Jasmonic Acid-Induced β-Cyclocitral Confers Resistance to Bacterial Blight and Negatively Affects Abscisic Acid Biosynthesis in Rice.International journal of molecular sciences · 2023Article
- Predicting and Visualizing Citrus Color Transformation Using a Deep Mask-Guided Generative Network.Plant phenomics (Washington, D.C.) · 2023Article
- Gardenia carotenoid cleavage dioxygenase 4a is an efficient tool for biotechnological production of crocins in green and non-green plant tissues.Plant biotechnology journal · 2022Article
- β-Cyclocitral: Emerging Bioactive Compound in Plants.Molecules (Basel, Switzerland) · 2022Review
15 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Citrus is the first tree crop in terms of fruit production. The colour of Citrus fruit is one of the main quality attributes, caused by the accumulation of carotenoids and their derivative C30 apocarotenoids, mainly β-citraurin (3-hydroxy-β-apo-8'-carotenal), which provide an attractive orange-reddish tint to the peel of oranges and Mandarins. Though carotenoid biosynthesis and its regulation have been extensively studied in Citrus fruits, little is known about the formation of C30 apocarotenoids. The aim of this study was to the identify carotenoid cleavage enzyme(s) [CCD(s)] involved in the peel-specific C30 apocarotenoids. In silico data mining revealed a new family of five CCD4-type genes in Citrus. One gene of this family, CCD4b1, was expressed in reproductive and vegetative tissues of different Citrus species in a pattern correlating with the accumulation of C30 apocarotenoids. Moreover, developmental processes and treatments which alter Citrus fruit peel pigmentation led to changes of β-citraurin content and CCD4b1 transcript levels. These results point to the involvement of CCD4b1 in β-citraurin formation and indicate that the accumulation of this compound is determined by the availability of the presumed precursors zeaxanthin and β-cryptoxanthin. Functional analysis of CCD4b1 by in vitro assays unequivocally demonstrated the asymmetric cleavage activity at the 7',8' double bond in zeaxanthin and β-cryptoxanthin, confirming its role in C30 apocarotenoid biosynthesis. Thus, a novel plant carotenoid cleavage activity targeting the 7',8' double bond of cyclic C40 carotenoids has been identified. These results suggest that the presented enzyme is responsible for the biosynthesis of C30 apocarotenoids in Citrus which are key pigments in fruit coloration.
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