ArticleJournal of clinical microbiology2013
MALDI biotyper-based rapid resistance detection by stable-isotope labeling.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed.
- Verification and application of an automated real-time antimicrobial susceptibility testing system: for accelerated antibiotic susceptibility testing and high-throughput screening of antibiotic synergistic effects.Journal of biological engineering · 2026Article
- Spectral Precision: Recent Advances in Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Pathogen Detection and Resistance Profiling.Microorganisms · 2025Review
- Stable isotope labeling as a promising tool for rapid drug susceptibility testing in Neisseria gonorrhoeae.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Evaluation of an adapted method of relative growth to determine the susceptibility of Enterobacterales to polymyxin B by MALDI-TOF MS.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Clinical microbiology in detection and identification of emerging microbial pathogens: past, present and future.Emerging microbes & infections · 2022Article
- Antimicrobial Susceptibility Testing: A Comprehensive Review of Currently Used Methods.Antibiotics (Basel, Switzerland) · 2022Review
- Methodology Establishment and Application of VITEK Mass Spectrometry to Detect Carbapenemase-ProducingFrontiers in cellular and infection microbiology · 2022Article
- Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Antimicrobial Susceptibility Testing.Journal of clinical microbiology · 2021Article
- Shortening the Time of the Identification and Antimicrobial Susceptibility Testing on Positive Blood Cultures with MALDI-TOF MS.Diagnostics (Basel, Switzerland) · 2021Article
- MALDI-TOF Mass Spectrometry Technology as a Tool for the Rapid Diagnosis of Antimicrobial Resistance in Bacteria.Antibiotics (Basel, Switzerland) · 2021Review
- Recent Development of Rapid Antimicrobial Susceptibility Testing Methods through Metabolic Profiling of Bacteria.Antibiotics (Basel, Switzerland) · 2021Review
- Approaches for characterizing and tracking hospital-associated multidrug-resistant bacteria.Cellular and molecular life sciences : CMLS · 2021Review
- Emerging Options for the Diagnosis of Bacterial Infections and the Characterization of Antimicrobial Resistance.International journal of molecular sciences · 2021Review
- The synergetic effect of Imipenem-clarithromycin combination in the Mycobacteroides abscessus complex.BMC microbiology · 2020Article
- Detection and identification of a protein biomarker in antibiotic-resistant Escherichia coli using intact protein LC offline MALDI-MS and MS/MS.Journal of applied microbiology · 2020Article
- Detection of Antibiotic-Resistance by MALDI-TOF Mass Spectrometry: An Expanding Area.Frontiers in cellular and infection microbiology · 2020Review
- Current status of MALDI-TOF mass spectrometry in clinical microbiology.Journal of food and drug analysis · 2019Review
- Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for the Rapid Detection of Antimicrobial Resistance Mechanisms and Beyond.Clinical microbiology reviews · 2019Review
- One System for All: Is Mass Spectrometry a Future Alternative for Conventional Antibiotic Susceptibility Testing?Frontiers in microbiology · 2019Review
- Rapid Detection of Extended-Spectrum β-Lactamases (ESBL) and AmpC β-Lactamases inFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Against the background of increasing numbers of resistant microorganisms, the fast and cost-efficient detection of microbial resistance is an important clinical requirement for optimal therapeutic intervention. Current routine assays take at least 5 h, but in most cases an overnight incubation is necessary to identify resistant isolates. The usage of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) profiling in combination with growth media containing isotopically labeled amino acids facilitates the detection of resistant microorganisms after 3 h or less directly from the profile spectrum. Growing microorganisms incorporate isotopically labeled amino acids, increasing protein masses and thereby leading to mass shifts of their corresponding peaks in the profile spectra. In the presence of antibiotics, only resistant microorganisms are able to grow and to incorporate the labeled amino acids. This leads to a difference in the mass spectra of susceptible and resistant isolates, allowing their differentiation. In the presented study, we demonstrated the applicability of this novel approach for the detection of methicillin-resistant Staphylococcus aureus and tested different bioinformatics approaches for automated data interpretation.
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