ArticleBMC genomics2013
Comparative analysis of the complete genome of an epidemic hospital sequence type 203 clone of vancomycin-resistant Enterococcus faecium.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Recombination and accessory genome turnover shaped the rise and decline of vanA Enterococcus faecium ST80 in Denmark.Genome medicine · 2026Article
- Clade-specific evolution and genome plasticity underlying antimicrobial resistance in Enterococcus faecium.Archives of microbiology · 2026Review
- Phenotypic and genomic analysis of the emerging poultry pathogenMicrobial genomics · 2025Article
- Characterisation ofJAC-antimicrobial resistance · 2025Article
- Genomic epidemiology of vancomycin-resistant Enterococcus faecium in Eastern Denmark from 2020 to 2022, and identification of vanB Tn1549 insertion sites.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- Deciphering vancomycin resistance inFrontiers in microbiology · 2025Article
- Enterococcus faecium: evolution, adaptation, pathogenesis and emerging therapeutics.Nature reviews. Microbiology · 2024Review
- Dissemination of Enterococcal Genetic Lineages: A One Health Perspective.Antibiotics (Basel, Switzerland) · 2023Review
- Using Genomics To Investigate an Outbreak of Vancomycin-Resistant Enterococcus faecium ST78 at a Large Tertiary Hospital in Queensland.Microbiology spectrum · 2023Article
- Multisite Detection of TnAntimicrobial agents and chemotherapy · 2023Article
- Emergence and Transmission of Daptomycin and Vancomycin-Resistant Enterococci Between Patients and Hospital Rooms.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Centre-specific bacterial pathogen typing affects infection-control decision making.Microbial genomics · 2021Article
- Genomic rearrangements uncovered by genome-wide co-evolution analysis of a major nosocomial pathogen,Microbial genomics · 2020Article
- Enterococcus faecium genome dynamics during long-term asymptomatic patient gut colonization.Microbial genomics · 2019Article
- Complete Genome Sequence of Enterococcus faecium QU50, a Thermophilic Lactic Acid Bacterium Capable of Metabolizing Xylose.Microbiology resource announcements · 2019Article
- Detection of vancomycin-resistantGenome research · 2019Article
- The Enterococcus: a Model of Adaptability to Its Environment.Clinical microbiology reviews · 2019Review
- Emergence of an Australian-like pstS-null vancomycin resistant Enterococcus faecium clone in Scotland.PloS one · 2019Article
- Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan.BMC microbiology · 2018Article
- Complete Genome Sequences of Isolates ofGenome announcements · 2017Article
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11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn this report we have explored the genomic and microbiological basis for a sustained increase in bloodstream infections at a major Australian hospital caused by Enterococcus faecium multi-locus sequence type (ST) 203, an outbreak strain that has largely replaced a predecessor ST17 sequence type.
resultsTo establish a ST203 reference sequence we fully assembled and annotated the genome of Aus0085, a 2009 vancomycin-resistant Enterococcus faecium (VREfm) bloodstream isolate, and the first example of a completed ST203 genome. Aus0085 has a 3.2 Mb genome, comprising a 2.9 Mb circular chromosome and six circular plasmids (2 kb-130 kb). Twelve percent of the 3222 coding sequences (CDS) in Aus0085 are not present in ST17 E. faecium Aus0004 and ST18 E. faecium TX16. Extending this comparison to an additional 12 ST17 and 14 ST203 E. faecium hospital isolate genomes revealed only six genomic regions spanning 41 kb that were present in all ST203 and absent from all ST17 genomes. The 40 CDS have predicted functions that include ion transport, riboflavin metabolism and two phosphotransferase systems. Comparison of the vancomycin resistance-conferring Tn1549 transposon between Aus0004 and Aus0085 revealed differences in transposon length and insertion site, and van locus sequence variation that correlated with a higher vancomycin MIC in Aus0085. Additional phenotype comparisons between ST17 and ST203 isolates showed that while there were no differences in biofilm-formation and killing of Galleria mellonella, ST203 isolates grew significantly faster and out-competed ST17 isolates in growth assays.
conclusionsHere we have fully assembled and annotated the first ST203 genome, and then characterized the genomic differences between ST17 and ST203 E. faecium. We also show that ST203 E. faecium are faster growing and can out-compete ST17 E. faecium. While a causal genetic basis for these phenotype differences is not provided here, this study revealed conserved genetic differences between the two clones, differences that can now be tested to explain the molecular basis for the success and emergence of ST203 E. faecium.
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