ArticleReproductive biology and endocrinology : RB&E2013
Proteomic analysis of seminal fluid from men exhibiting oxidative stress.
Article in Reproductive biology and endocrinology : RB&E, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 106 citations in OpenAlex.
- Spinal Cord Injury-Related Male Infertility: Oxidative Stress, Inflammation, and Sperm DNA Damage.Journal of clinical medicine · 2026Review
- CD43 expressed in Sertoli, Leydig and germ cells inhibits testicular function and represents a potential target to treat male infertility or effect male contraception.Reproductive biology and endocrinology : RB&E · 2025Article
- Dissecting the genetic association between abnormal sperm parameters and depression: a transcriptome-wide analysis of 157 participants.Journal of assisted reproduction and genetics · 2025Article
- Intraindividual variability of semen quality, proteome, and sncRNA profiles in a healthy cohort of young adults.Andrology · 2025Article
- Proteomic Analysis of Fibroblasts Exposed to Resin Composite Release.Proteomics. Clinical applications · 2025Article
- Proteomics and Metabolomics in Varicocele-Associated Male Infertility: Advancing Precision Diagnostics and Therapy.Journal of clinical medicine · 2024Review
- The protective effects of antioxidants against endogenous and exogenous oxidative stress on bull sperm.In vitro cellular & developmental biology. Animal · 2024Article
- A Comparison of White and Yellow Seminal Plasma Phosphoproteomes Obtained from Turkey (International journal of molecular sciences · 2024Article
- Oxidative Stress-Associated Male Infertility: Current Diagnostic and Therapeutic Approaches.Medicina (Kaunas, Lithuania) · 2024Review
- Proteomic approach towards identification of seminal fluid biomarkers from individuals with severe oligozoospermia, cryptozoospermia and non-obstructive azoospermia: a pilot study.Translational andrology and urology · 2023Article
- Cessation of chronic delta-9-tetrahydrocannabinol use partially reverses impacts on male fertility and the sperm epigenome in rhesus macaques.Fertility and sterility · 2023Article
- Semen proteomics reveals alterations in fertility-related proteins post-recovery from COVID-19.Frontiers in physiology · 2023Article
- Unraveling the harmful effect of oxidative stress on male fertility: A mechanistic insight.Frontiers in endocrinology · 2023Review
- Relationships between Biomarkers of Oxidative Stress in Seminal Plasma and Sperm Motility in Bulls before and after Cryopreservation.Animals : an open access journal from MDPI · 2022Article
- Transcriptome profiling and proteomic validation reveals targets of the androgen receptor signaling in the BT-474 breast cancer cell line.Clinical proteomics · 2022Article
- Omics and Male Infertility: Highlighting the Application of Transcriptomic Data.Life (Basel, Switzerland) · 2022Review
- Molecular Interactions Associated with Oxidative Stress-Mediated Male Infertility: Sperm and Seminal Plasma Proteomics.Advances in experimental medicine and biology · 2022Review
- Parkinson Disease Protein 7 (PARK7) Is Related to the Ability of Mammalian Sperm to Undergo In Vitro Capacitation.International journal of molecular sciences · 2021Article
- Proteomic Profiling of Seminal Plasma Proteins in Varicocele Patients.The world journal of men's health · 2021Article
- Seminal Plasma Proteomic Biomarkers of Oxidative Stress.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSeminal plasma serves as a natural reservoir of antioxidants. It helps to remove excessive formation of reactive oxygen species (ROS) and consequently, reduce oxidative stress. Proteomic profiling of seminal plasma proteins is important to understand the molecular mechanisms underlying oxidative stress and sperm dysfunction in infertile men.
methodsThis prospective study consisted of 52 subjects: 32 infertile men and 20 healthy donors. Once semen and oxidative stress parameters were assessed (ROS, antioxidant concentration and DNA damage), the subjects were categorized into ROS positive (ROS+) or ROS negative (ROS-). Seminal plasma from each group was pooled and subjected to proteomics analysis. In-solution digestion and protein identification with liquid chromatography tandem mass spectrometry (LC-MS/MS), followed by bioinformatics analyses was used to identify and characterize potential biomarker proteins.
resultsA total of 14 proteins were identified in this analysis with 7 of these common and unique proteins were identified in both the ROS+ and ROS- groups through MASCOT and SEQUEST analyses, respectively. Prolactin-induced protein was found to be more abundantly present in men with increased levels of ROS. Gene ontology annotations showed extracellular distribution of proteins with a major role in antioxidative activity and regulatory processes.
conclusionsWe have identified proteins that help protect against oxidative stress and are uniquely present in the seminal plasma of the ROS- men. Men exhibiting high levels of ROS in their seminal ejaculate are likely to exhibit proteins that are either downregulated or oxidatively modified, and these could potentially contribute to male infertility.
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