ArticleBMC veterinary research2013
Development and validation of TaqMan probe based real time PCR assays for the specific detection of genotype A and B small ruminant lentivirus strains.
Article in BMC veterinary research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- Identification of lineage-associated polymorphisms in the ROP18 3' flanking region and development of molecular assays for differentiation ofFrontiers in microbiology · 2026Article
- Distribution of Small Ruminant Lentivirus Genotypes A and B in Goat and Sheep Production Units in Mexico.Veterinary sciences · 2025Article
- Development and Evaluation of a Candidate Inactivated Vaccine Against Bluetongue Virus Serotype 4 (BTV4).Vaccines · 2024Article
- Comparison of serological and molecular methods for differentiation between genotype A and genotype B strains of small ruminant lentiviruses.Journal of veterinary research · 2024Article
- Evaluation of Serological Methods and a New Real-Time Nested PCR for Small Ruminant Lentiviruses.Pathogens (Basel, Switzerland) · 2022Article
- Serological, Molecular and Culture-Based Diagnosis of Lentiviral Infections in Small Ruminants.Viruses · 2021Review
- Accurate Diagnosis of Small Ruminant Lentivirus Infection Is Needed for Selection of Resistant Sheep through TMEM154 E35K Genotyping.Pathogens (Basel, Switzerland) · 2021Article
- Ovine Progressive Pneumonia: Diagnosis and Seroprevalence in the South of Sonora, Mexico.Case reports in veterinary medicine · 2021Article
- Multi-Platform Detection of Small Ruminant Lentivirus Antibodies and Provirus as Biomarkers of Production Losses.Frontiers in veterinary science · 2020Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSmall ruminant lentiviruses (SRLV) are members of the Retroviridae family and infect goats and sheep worldwide. Detection of specific antibodies using AGID and ELISA is the most commonly used means of diagnosing SRLV infection. The most frequent molecular method for detecting the provirus genome is PCR, using peripheral blood leucocytes as target cells. Real time PCR has also recently been used. The aim of this study was to develop a real time PCR for detection of SRLV in order to improve molecular diagnostics of SRLV infections in sheep and goats.
resultsTwo new real time PCR assays using TaqMan probes for the specific detection of genotype A (MVV assay) and genoptype B (CAEV assay) SRLV strains and differentiation between them were developed and validated at both analytical and diagnostic levels following MIQE guidelines. The validation results showed that the new real time PCR is 100% specific, with a reliable limit of detection of 26 (CAEV assay) and 72 (MVV assay) plasmid DNA copies, while compared to ELISA the diagnostic sensitivity of both assays was 79% when tested with Slovenian SRLV field samples. Intra-assay and inter-assay coefficients of variation showed overall good repeatability and reproducibility of the new real time PCR assays, except for the highest dilutions.
conclusionsTwo new TaqMan probe based real time PCR assays for the specific detection of genotype A and B SRLV strains and differentiation between them were developed and validated. They can serve as an additional tool for confirming infection with SRLV and may also be useful for early detection of infected animals prior to seroconversion.
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