ArticleACS nano2013
Dielectrophoretic sorting of membrane protein nanocrystals.
Article in ACS nano, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- When to Use Rectangular Waveforms in Dielectrophoresis Application to Increase Separation and Sorting Efficiency.Electrophoresis · 2025Article
- High-Throughput Continuous Free-Flow Dielectrophoretic Trapping of Micron-Scale Particles and Cells in Paper Using Localized Nonuniform Pore-Scale-Generated Paper-Based Electric Field Gradients.Analytical chemistry · 2024Article
- Microfluidic Isolation and Enrichment of Nanoparticles.ACS nano · 2020Review
- Charge-Based Separation of Micro- and Nanoparticles.Micromachines · 2020Article
- Protein-crystal detection with a compact multimodal multiphoton microscope.Communications biology · 2020Article
- Electrokinetic characterization of synthetic protein nanoparticles.Beilstein journal of nanotechnology · 2020Article
- 3D printed droplet generation devices for serial femtosecond crystallography enabled by surface coating.Journal of applied crystallography · 2019Article
- Isolation and identification of Listeria monocytogenes utilizing DC insulator-based dielectrophoresis.Analytica chimica acta · 2019Article
- On the Impact of Electrostatic Correlations on the Double-Layer Polarization of a Spherical Particle in an Alternating Current Field.Langmuir : the ACS journal of surfaces and colloids · 2018Article
- Three-Dimensional Reservoir-Based Dielectrophoresis (rDEP) for Enhanced Particle Enrichment.Micromachines · 2018Article
- Assessment of Sub-Micron Particles by Exploiting Charge Differences with Dielectrophoresis.Micromachines · 2017Article
- Refinement of insulator-based dielectrophoresis.The Analyst · 2017Article
- Dielectrophoresis for Biomedical Sciences Applications: A Review.Sensors (Basel, Switzerland) · 2017Review
- On utilizing alternating current-flow field effect transistor for flexibly manipulating particles in microfluidics and nanofluidics.Biomicrofluidics · 2016Article
- Microfluidic sorting of protein nanocrystals by size for X-ray free-electron laser diffraction.Structural dynamics (Melville, N.Y.) · 2015Article
- Development of the resolution theory for gradient insulator-based dielectrophoresis.Electrophoresis · 2015Article
- High throughput protein nanocrystal fractionation in a microfluidic sorter.Analytical chemistry · 2015Article
- Protein dielectrophoresis and the link to dielectric properties.Bioanalysis · 2015Review
- Temporal and spatial temperature measurement in insulator-based dielectrophoretic devices.Analytical chemistry · 2014Article
- Insulator-based dielectrophoresis of mitochondria.Biomicrofluidics · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Structure elucidation of large membrane protein complexes is still a considerable challenge, yet is a key factor in drug development and disease combat. Femtosecond nanocrystallography is an emerging technique with which structural information of membrane proteins is obtained without the need to grow large crystals, thus overcoming the experimental riddle faced in traditional crystallography methods. Here, we demonstrate for the first time a microfluidic device capable of sorting membrane protein crystals based on size using dielectrophoresis. We demonstrate the excellent sorting power of this new approach with numerical simulations of selected submicrometer beads in excellent agreement with experimental observations. Crystals from batch crystallization broths of the huge membrane protein complex photosystem I were sorted without further treatment, resulting in a high degree of monodispersity and crystallinity in the ~100 nm size range. Microfluidic integration, continuous sorting, and nanometer-sized crystal fractions make this method ideal for direct coupling to femtosecond nanocrystallography.
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Registered trials
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