ArticleAnnals of laboratory medicine2013
The combination of real-time PCR and HPLC for the identification of non-tuberculous mycobacteria.
Article in Annals of laboratory medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Visible label-free detection of bacterial DNA using flocculation of sterically stabilised cationic latexes.Journal of materials chemistry. B · 2023Article
- Determination of antibiotic resistance and high-performance liquid chromatography profiles for Mycobacterium species.Journal of clinical laboratory analysis · 2018Article
- Direct identification ofExperimental and therapeutic medicine · 2014Article
- Distribution of nontuberculous mycobacteria by multigene sequence-based typing and clinical significance of isolated strains.Journal of clinical microbiology · 2014Article
- Highlight on advances in nontuberculous mycobacterial disease in North America.BioMed research international · 2014Review
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used HPLC and AdvanSure real-time PCR (LG Life Sciences, Korea) to retrospectively analyze non-tuberculous mycobacteria (NTM) in 133 clinical specimens. The specimens were culture-positive for NTM and the HPLC method identified 130 strains of mycobacteria from the cultures (97.7%) at the species level. Among the isolates, 48 Mycobacterium. kansasii (36.1%), 39 M. intracellulare (29.3%), 17 M. avium (12.8%), 16 M. abscessus (12.0%), 6 M. fortuitum (4.5%), 2 M. szulgai (1.5%), 2 M. gordonae (1.5%), and 3 unclassified NTM strains (2.3%) were identified. The real-time PCR assay identified 60 NTM-positive specimens (45.1%), 65 negative specimens (48.9%), and 8 M. tuberculosis (TB)-positive specimens (6.0%). The real-time PCR assay is advantageous because of its rapid identification of NTM. However, in our study, the real-time PCR assay showed relatively low sensitivity (45.1%) when using direct specimens including sputum and bronchoalveolar lavage (BAL) fluid. HPLC is useful as it discriminates NTM at the species level, although it is time-consuming and requires specific equipment and technical expertise. A combination of both methods will be helpful for the rapid and accurate identification of mycobacteria in clinical laboratories.
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