ArticleThe Journal of biological chemistry2013
Genetic code-guided protein synthesis and folding in Escherichia coli.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- How soluble misfolded proteins bypass chaperones at the molecular level.Nature communications · 2023Pooled it
- Translation Rates and Protein Folding.Journal of molecular biology · 2024Review
- Impact of the chemical modification of tRNAs anticodon loop on the variability and evolution of codon usage in proteobacteria.Frontiers in microbiology · 2024Article
- A BioBricks Metabolic Engineering Platform for the Biosynthesis of Anthracyclinones inACS synthetic biology · 2022Article
- Application of Statistical Learning to Identify Omicron Mutations in SARS-CoV-2 Viral Genome Sequence Data From Populations in Africa and the United States.JAMA network open · 2022Article
- Mutations in viral nucleocapsid protein and endoRNase are discovered to associate with COVID19 hospitalization risk.Scientific reports · 2022Article
- Oxidative stress strongly restricts the effect of codon choice on the efficiency of protein synthesis inFrontiers in microbiology · 2022Article
- A Code Within a Code: How Codons Fine-Tune Protein Folding in the Cell.Biochemistry. Biokhimiia · 2021Review
- Article
- Codon usage revisited: Lack of correlation between codon usage and the number of tRNA genes in enterobacteria.Biochemical and biophysical research communications · 2018Article
- Plasmodium apicoplast tyrosyl-tRNA synthetase recognizes an unusual, simplified identity set in cognate tRNATyr.PloS one · 2018Article
- Predicting synonymous codon usage and optimizing the heterologous gene for expression in E. coli.Scientific reports · 2017Article
- Effect of DNA sequence of Fab fragment on yield characteristics and cell growth of E. coli.Scientific reports · 2017Article
- Fast Protein Translation Can Promote Co- and Posttranslational Folding of Misfolding-Prone Proteins.Biophysical journal · 2017Article
- High yields and soluble expression of superoxide dismutases in Escherichia coli due to the HIV-1 Tat peptide via increases in mRNA transcription.Experimental & molecular medicine · 2016Article
- The Yin and Yang of codon usage.Human molecular genetics · 2016Review
- The ribosome in action: Tuning of translational efficiency and protein folding.Protein science : a publication of the Protein Society · 2016Review
- Coevolution mechanisms that adapt viruses to genetic code variations implemented in their hosts.Journal of genetics · 2016Article
- Synonymous Codons Direct Cotranslational Folding toward Different Protein Conformations.Molecular cell · 2016Article
- Cotranslational protein folding reveals the selective use of synonymous codons along the coding sequence of a low expression gene.Journal of genetics · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Universal genetic codes are degenerated with 61 codons specifying 20 amino acids, thus creating synonymous codons for a single amino acid. Synonymous codons have been shown to affect protein properties in a given organism. To address this issue and explore how Escherichia coli selects its "codon-preferred" DNA template(s) for synthesis of proteins with required properties, we have designed synonymous codon libraries based on an antibody (scFv) sequence and carried out bacterial expression and screening for variants with altered properties. As a result, 342 codon variants have been identified, differing significantly in protein solubility and functionality while retaining the identical original amino acid sequence. The soluble expression level varied from completely insoluble aggregates to a soluble yield of ~2.5 mg/liter, whereas the antigen-binding activity changed from no binding at all to a binding affinity of > 10(-8) m. Not only does our work demonstrate the involvement of genetic codes in regulating protein synthesis and folding but it also provides a novel screening strategy for producing improved proteins without the need to substitute amino acids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.