ArticleCold Spring Harbor protocols2013
Patch-clamp electrophysiology of intracellular Ca2+ channels.
Article in Cold Spring Harbor protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 23 citations in OpenAlex.
- Pharmacological potentiators of the calcium signaling cascade identified by high-throughput screening.PNAS nexus · 2023Article
- Fluorescent indicators for imaging membrane potential of organelles.Current opinion in chemical biology · 2022Review
- Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters.Cells · 2022Review
- Semiconducting Carbon Nanotube-Based Nanodevices for Monitoring the Effects of Chlorphenamine on the Activities of Intracellular CaJournal of analytical methods in chemistry · 2022Article
- KRAP tethers IPNature communications · 2021Article
- Article
- ER-luminal [CaeLife · 2020Article
- Pharmacological modulation of mitochondrial ion channels.British journal of pharmacology · 2019Review
- The acidocalcisome inositol-1,4,5-trisphosphate receptor ofThe Journal of biological chemistry · 2019Article
- TraceSpecks: A Software for Automated Idealization of Noisy Patch-Clamp and Imaging Data.Biophysical journal · 2018Article
- InsPBMC biology · 2018Article
- Analyzing optical imaging of CaCell calcium · 2017Article
- Characterization of Two-Pore Channel 2 by Nuclear Membrane Electrophysiology.Scientific reports · 2016Article
- Organellar channels and transporters.Cell calcium · 2015Review
- Inositol 1,4,5-trisphosphate receptors in the endoplasmic reticulum: A single-channel point of view.Cell calcium · 2015Review
- Monovalent cationic channel activity in the inner membrane of nuclei from skeletal muscle fibers.Biophysical journal · 2014Article
- General anesthetic isoflurane modulates inositol 1,4,5-trisphosphate receptor calcium channel opening.Anesthesiology · 2014Article
- Characterization of ryanodine receptor type 1 single channel activity using "on-nucleus" patch clamp.Cell calcium · 2014Article
- Bilayer measurement of endoplasmic reticulum Ca2+ channels.Cold Spring Harbor protocols · 2013Article
- Nuclear patch-clamp electrophysiology of Ca2+ channels.Cold Spring Harbor protocols · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The modulation of cytoplasmic free Ca(2+) concentration ([Ca(2+)]i) is a universal intracellular signaling pathway that regulates numerous cellular physiological processes. Ubiquitous intracellular Ca(2+)-release channels localized to the endoplasmic/sarcoplasmic reticulum-inositol 1,4,5-trisphosphate receptor (InsP3R) and ryanodine receptor (RyR) channels-play a central role in [Ca(2+)]i signaling in all animal cells. Despite their intracellular localization, electrophysiological studies of the single-channel permeation and gating properties of these Ca(2+)-release channels using the powerful patch-clamp approach have been possible by application of this technique to isolated nuclei because the channels are present in membranes of the nuclear envelope. Here we provide a concise description of how nuclear patch-clamp experiments have been used to study single-channel properties of different InsP3R channels in the outer nuclear membrane. We compare this with other methods for studying intracellular Ca(2+) release. We also briefly describe application of the technique to InsP3R channels in the inner nuclear membrane and to channels in the outer nuclear membrane of HEK293 cells expressing recombinant RyR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.