ArticleProceedings of the National Academy of Sciences of the United States of America2013
Diagnosis of tumors during tissue-conserving surgery with integrated autofluorescence and Raman scattering microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers, 1 of them a synthesis that pooled it.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
92 citing papers in PubMed, 1 synthesis or guideline pooled it, 236 citations in OpenAlex.
- Pooled it
- Label-free optical biomarker for prostate cancer diagnosis.Molecular medicine (Cambridge, Mass.) · 2026Article
- Spatially multiplexed random-access Raman probe for in vivo tissue measurements.Biomedical optics express · 2026Article
- Feasibility of diffuse Raman spectroscopy to detect in-vivo molecular changes in the tissue induced by subcutaneous implants.Biomedical optics express · 2025Article
- Review
- Development of a rapid and novel diagnostic technique for cardiac amyloidosis using Raman spectroscopy.Research square · 2025Article
- Hyperspectral oblique plane microscopy enables spontaneous, label-free imaging of biological dynamic processes in live animals.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Intraoperative spectroscopic evaluation of sentinel lymph nodes in breast cancer surgery.Breast cancer research and treatment · 2024Article
- Inspiring a convergent engineering approach to measure and model the tissue microenvironment.Heliyon · 2024Review
- Correlative Raman Imaging: Development and Cancer Applications.Biosensors · 2024Review
- Non-invasive detection of regulatory T cells with Raman spectroscopy.Scientific reports · 2024Article
- Opto-Lipidomics of Tissues.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Portable System for In-Clinic Differentiation of Skin Cancers from Benign Skin Lesions and Inflammatory Dermatoses.JID innovations : skin science from molecules to population health · 2024Article
- Unraveling Molecular Composition in Biological Samples-Systematic Evaluation of Statistical Methods for the Analysis of Hyperspectral Raman Data.Analytical chemistry · 2023Article
- Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model.Biomedical optics express · 2023Article
- From Vibrations to Visions: Raman Spectroscopy's Impact on Skin Cancer Diagnostics.Journal of clinical medicine · 2023Review
- Multimodal imaging of metabolic activities for distinguishing subtypes of breast cancer.Biomedical optics express · 2023Article
- Comparison of denoising tools for the reconstruction of nonlinear multimodal images.Biomedical optics express · 2023Article
- Molecular Fingerprint Detection Using Raman and Infrared Spectroscopy Technologies for Cancer Detection: A Progress Review.Biosensors · 2023Review
- High-throughput line-illumination Raman microscopy with multislit detection.Biomedical optics express · 2023Article
32 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Tissue-conserving surgery is used increasingly in cancer treatment. However, one of the main challenges in this type of surgery is the detection of tumor margins. Histopathology based on tissue sectioning and staining has been the gold standard for cancer diagnosis for more than a century. However, its use during tissue-conserving surgery is limited by time-consuming tissue preparation steps (1-2 h) and the diagnostic variability inherent in subjective image interpretation. Here, we demonstrate an integrated optical technique based on tissue autofluorescence imaging (high sensitivity and high speed but low specificity) and Raman scattering (high sensitivity and high specificity but low speed) that can overcome these limitations. Automated segmentation of autofluorescence images was used to select and prioritize the sampling points for Raman spectroscopy, which then was used to establish the diagnosis based on a spectral classification model (100% sensitivity, 92% specificity per spectrum). This automated sampling strategy allowed objective diagnosis of basal cell carcinoma in skin tissue samples excised during Mohs micrographic surgery faster than frozen section histopathology, and one or two orders of magnitude faster than previous techniques based on infrared or Raman microscopy. We also show that this technique can diagnose the presence or absence of tumors in unsectioned tissue layers, thus eliminating the need for tissue sectioning. This study demonstrates the potential of this technique to provide a rapid and objective intraoperative method to spare healthy tissue and reduce unnecessary surgery by determining whether tumor cells have been removed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.