ArticleJournal of nuclear medicine : official publication, Society of Nuclear Medicine2013
High-resolution radioluminescence microscopy of 18F-FDG uptake by reconstructing the β-ionization track.
Article in Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 50 citations in OpenAlex.
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- Multiplexed Single-Cell Measurements of FDG Uptake and Lactate Release Using Droplet Microfluidics.Technology in cancer research & treatment · 2019Article
- Single-Cell Imaging Using Radioluminescence Microscopy Reveals Unexpected Binding Target for [18F]HFB.Molecular imaging and biology · 2018Article
- In silico optimization of radioluminescence microscopy.Journal of biophotonics · 2018Article
- Ultrahigh resolution radiation imaging system using an optical fiber structure scintillator plate.Scientific reports · 2018Article
- Toward a Droplet-Based Single-Cell Radiometric Assay.Analytical chemistry · 2017Article
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Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
unlabelledRadioluminescence microscopy is a new method for imaging radionuclide uptake by single live cells with a fluorescence microscope. Here, we report a particle-counting scheme that improves spatial resolution by overcoming the β-range limit.
methodsShort frames (10 μs-1 s) were acquired using a high-gain camera coupled to a microscope to capture individual ionization tracks. Optical reconstruction of the β-ionization track (ORBIT) was performed to localize individual β decays, which were aggregated into a composite image. The new approach was evaluated by imaging the uptake of (18)F-FDG in nonconfluent breast cancer cells.
resultsAfter image reconstruction, ORBIT resulted in better definition of individual cells. This effect was particularly noticeable in small clusters (2-4 cells), which occur naturally even for nonconfluent cell cultures. The annihilation and Bremsstrahlung photon background signal was markedly lower. Single-cell measurements of (18)F-FDG uptake that were computed from ORBIT images more closely matched the uptake of the fluorescent glucose analog (Pearson correlation coefficient, 0.54 vs. 0.44, respectively).
conclusionORBIT can image the uptake of a radiotracer in living cells with spatial resolution better than the β range. In principle, ORBIT may also allow for greater quantitative accuracy because the decay rate is measured more directly, with no dependency on the β-particle energy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.