ArticleNucleic acids research2013
E-TALEN: a web tool to design TALENs for genome engineering.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- biGMamAct: efficient CRISPR/Cas9-mediated docking of large functional DNA cargoes at theSynthetic biology (Oxford, England) · 2025Article
- Current State of Human Gene Therapy: Approved Products and Vectors.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Corneal gene therapy: Structural and mechanistic understanding.The ocular surface · 2023Article
- Genome-editing approaches and applications: a brief review on CRISPR technology and its role in cancer.3 Biotech · 2021Review
- Tools for experimental and computational analyses of off-target editing by programmable nucleases.Nature protocols · 2021Review
- A most formidable arsenal: genetic technologies for building a better mouse.Genes & development · 2020Review
- Programmable Molecular Scissors: Applications of a New Tool for Genome Editing in Biotech.Molecular therapy. Nucleic acids · 2019Review
- Precision gene editing technology and applications in nephrology.Nature reviews. Nephrology · 2018Review
- Hypoxia inducible factor HIF-1 promotes myeloid-derived suppressor cells accumulation through ENTPD2/CD39L1 in hepatocellular carcinoma.Nature communications · 2017Article
- TALEN gene editing takes aim on HIV.Human genetics · 2016Review
- Nuclease Target Site Selection for Maximizing On-target Activity and Minimizing Off-target Effects in Genome Editing.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Review
- Designed nucleases for targeted genome editing.Plant biotechnology journal · 2016Review
- Genome engineering of isogenic human ES cells to model autism disorders.Nucleic acids research · 2015Article
- Progress and prospects of engineered sequence-specific DNA modulating technologies for the management of liver diseases.World journal of hepatology · 2015Review
- TALEN-mediated genome engineering to generate targeted mice.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- TAL effectors: tools for DNA targeting.Briefings in functional genomics · 2014Article
- Tools for TAL effector design and target prediction.Methods (San Diego, Calif.) · 2014Review
- Targeted genome editing in Aedes aegypti using TALENs.Methods (San Diego, Calif.) · 2014Article
- TALEN and CRISPR/Cas Genome Editing Systems: Tools of Discovery.Acta naturae · 2014Article
- CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing.Nucleic acids research · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Use of transcription activator-like effector nucleases (TALENs) is a promising new technique in the field of targeted genome engineering, editing and reverse genetics. Its applications span from introducing knockout mutations to endogenous tagging of proteins and targeted excision repair. Owing to this wide range of possible applications, there is a need for fast and user-friendly TALEN design tools. We developed E-TALEN (http://www.e-talen.org), a web-based tool to design TALENs for experiments of varying scale. E-TALEN enables the design of TALENs against a single target or a large number of target genes. We significantly extended previously published design concepts to consider genomic context and different applications. E-TALEN guides the user through an end-to-end design process of de novo TALEN pairs, which are specific to a certain sequence or genomic locus. Furthermore, E-TALEN offers a functionality to predict targeting and specificity for existing TALENs. Owing to the computational complexity of many of the steps in the design of TALENs, particular emphasis has been put on the implementation of fast yet accurate algorithms. We implemented a user-friendly interface, from the input parameters to the presentation of results. An additional feature of E-TALEN is the in-built sequence and annotation database available for many organisms, including human, mouse, zebrafish, Drosophila and Arabidopsis, which can be extended in the future.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.