ArticleSurgical endoscopy2014
Real-time optical diagnosis for surgical margin in low rectal cancer using multiphoton microscopy.
Article in Surgical endoscopy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Margin diagnosis for endoscopic submucosal dissection of early gastric cancer using multiphoton microscopy.Surgical endoscopy · 2020Trial
- Pilot Clinical Evaluation of a Confocal Microlaparoscope for Ovarian Cancer Detection.International journal of gynecological cancer : official journal of the International Gynecological Cancer Society · 2016Trial
- Association of the tumor microenvironment collagen score and immunoscore with colon cancer lymph node metastasis.BMC cancer · 2025Article
- Towards next-generation diagnostic pathology: AI-empowered label-free multiphoton microscopy.Light, science & applications · 2024Review
- Label-free quantification of imaging features in the extracellular matrix of left and right-sided colon cancer tissues.Scientific reports · 2024Article
- Intraoperative imaging in pathology-assisted surgery.Nature biomedical engineering · 2022Review
- Triple-modality co-registered endoscope featuring wide-field reflectance imaging, and high-resolution multiphoton and optical coherence microscopy.Proceedings of SPIE--the International Society for Optical Engineering · 2021Article
- Pre-Clinical Translation of Second Harmonic Microscopy of Meniscal and Articular Cartilage Using a Prototype Nonlinear Microendoscope.IEEE journal of translational engineering in health and medicine · 2019Article
- Article
- Rapid diagnosis and intraoperative margin assessment of human lung cancer with fluorescence lifetime imaging microscopy.BBA clinical · 2017Article
- Pediatric oncologic endosurgery.International journal of surgery. Oncology · 2017Review
- Identifying the neck margin status of ductal adenocarcinoma in the pancreatic head by multiphoton microscopy.Scientific reports · 2017Article
- In vivo multiphoton tomography and fluorescence lifetime imaging of human brain tumor tissue.Journal of neuro-oncology · 2016Article
- Intraoperative Label-Free Multimodal Nonlinear Optical Imaging for Point-of-Procedure Cancer Diagnostics.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics SocietyArticle
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMultiphoton microscopy (MPM), based on advances in the field of nonlinear optics and femtosecond lasers, has been shown to provide detailed real-time information on tissue architecture and cell morphology in live tissue. The purpose of this study was to evaluate the feasibility of using MPM to make real-time optical diagnoses for surgical margins in low rectal cancers.
methodsThirty fresh, unfixed, and unstained full-thickness surgical margins of low rectal cancers underwent MPM examination and then went through intraoperative frozen procedures and routine pathological procedures. MPM images were compared with the gold standard hematoxylin-eosin (H-E) stained images.
resultsMPM images were acquired by two channels: broadband autofluorescence from cells and second harmonic generation (SHG) from tissue collagen. Peak multiphoton signal intensity was detected in mucosa excited at 800 nm. There were significant differences between negative surgical margins and positive surgical margins under MPM examination. In negative surgical margins, MPM revealed regular tissue architecture and cell morphology, including a typical foveolar pattern with central, round crypt openings, and glands lined by epithelial and goblet cells. SHG signals could be detected around the glands. In positive surgical margins, MPM demonstrated irregular tubular structures, reduced stroma, and cellular and nuclear pleomorphisms. Cancer cells were characterized by an irregular size and shape, enlarged nuclei, and an increased nuclear-cytoplasmic ratio. SHG signals were significantly decreased in positive surgical margins compared with negative surgical margins. MPM images were comparable to H-E stained images.
conclusionsWe demonstrated the feasibility of using MPM to make real-time optical diagnoses for surgical margins in low rectal cancer. With the miniaturization and integration of colonoscopy or probes, MPM has the potential to provide real-time noninvasive optical diagnosis for surgical margins in low rectal cancer in the near future.
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