ArticleMolecular biotechnology2014
Genetic improvement of sugarcane for drought and salinity stress tolerance using Arabidopsis vacuolar pyrophosphatase (AVP1) gene.
Article in Molecular biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 111 citations in OpenAlex.
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- Breeding innovations focused on drought tolerance, and Nitrogen and phosphorus uptake, for sustainable and climate-resilient sugarcane.Frontiers in plant science · 2026Review
- UBIQUITIN-CONJUGATING ENZYME34 mediates pyrophosphatase AVP1 turnover and regulates abiotic stress responses in Arabidopsis.Plant physiology · 2025Article
- Enhancing quality and climate resilient traits in vegetatively propagated polyploids: transgenic and genome editing advancements, challenges and future directions.Frontiers in genetics · 2025Review
- The role of gibberellin synthase gene VvGA2ox7 acts as a positive regulator to salt stress in Arabidopsis thaliana.BMC plant biology · 2024Article
- Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses.Plants (Basel, Switzerland) · 2024Review
- Coordination of mPlants (Basel, Switzerland) · 2023Article
- Root and Leaf Anatomy, Ion Accumulation, and Transcriptome Pattern under Salt Stress Conditions in Contrasting Genotypes ofPlants (Basel, Switzerland) · 2023Article
- New methods for sorghum transformation in temperate climates.AoB PLANTS · 2023Review
- Reasons and riddance of Agrobacterium tumefaciens overgrowth in plant transformation.Transgenic research · 2023Review
- Drought and salinity stresses induced physio-biochemical changes in sugarcane: an overview of tolerance mechanism and mitigating approaches.Frontiers in plant science · 2023Review
- Genetic engineering: an efficient approach to mitigating biotic and abiotic stresses in sugarcane cultivation.Plant signaling & behavior · 2022Article
- Overexpression ofPlants (Basel, Switzerland) · 2022Article
- Genetic manipulation for abiotic stress resistance traits in crops.Frontiers in plant science · 2022Review
- A short review on sugarcane: its domestication, molecular manipulations and future perspectives.Genetic resources and crop evolution · 2022Review
- Establishment of an efficientMolecular breeding : new strategies in plant improvement · 2021Article
- Effects of Seaweed Extracts on the Growth, Physiological Activity, Cane Yield and Sucrose Content of Sugarcane in China.Frontiers in plant science · 2021Article
- Genetic Transformation of Sugarcane, Current Status and Future Prospects.Frontiers in plant science · 2021Review
- Current breeding and genomic approaches to enhance the cane and sugar productivity under abiotic stress conditions.3 Biotech · 2020Review
- Phenotypic characterization ofApplications in plant sciences · 2020Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sugarcane plant is a glycophyte, hence its growth and sucrose contents are severely affected by drought and salinity stresses. Bioengineering approaches offer a plausible and rapid solution to mitigate these losses. Therefore for genetic improvement of sugarcane against these stresses, the present study was conceived to transform Arabidopsis Vacuolar Pyrophosphatase (AVP1) gene--confers tolerance against drought and salinity--into sugarcane through Agrobacterium. For this purpose, highly regenerable apical buds of sugarcane variety CP77-400 were used as explants. EHA105 strain of Agrobacterium harboring pGreen0029 vector containing AVP1 gene driven under 35SCaMV promoter was employed for transformation. The key factors studied include application of acetosyringone, cefotaxime, kanamycin, and co-cultivation period for successful transformation. Maximum regeneration frequency of 77.5 % was achieved on MS media containing 1 mg/l BAP, 1 mg/l Kn, 1 mg/l GA₃, 0.25 mg/l NAA, 50 μM acetosyringone, 500 mg/l cefotaxime, and 150 mg/l kanamycin on 3 days of co-cultivation. The results revealed that apical buds are distinctive viable tissues for sugarcane transformation and regeneration to produce a large number of CP77-400 transgenic plants in shorter period of time without intervening mosaics and chimeras. The AVP1 transcripts expression in transgenic lines at various levels was detected by RT-PCR. Longer and profuse root system was observed in transgenic plants in comparison with control plants. Concomitantly, only transgenic plants were able to withstand higher NaCl salt stress as well as scarcity of water thus, showing tolerance against salinity and drought stresses.
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